Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University, Fukushima, Japan.
Transfusion. 2013 Oct;53(10):2293-8. doi: 10.1111/trf.12088. Epub 2013 Jan 24.
Microparticles in blood components might contribute to transfusion-related immunomodulation or other side effects. To elucidate the role of leukofiltration, we compared three commercially available filters for their effect on platelet (PLT)-derived (PDMP), leukocyte-derived (LDMP), and red blood cell-derived (RDMP) microparticle formation in apheresis PLTs.
Apheresis PLTs from pairs of ABO-identical male donors were pooled and divided into four volumes. One volume was stored without filtration, whereas the other three were filtered with different devices. PDMPs, LDMPs, and RDMPs were measured by flow cytometry during 2 weeks of controlled-temperature (22°C) agitated storage.
On average, PDMPs doubled over 5 days of storage, followed by a much steeper increase by which PDMPs on Day 14 were nearly 20 times higher than on Day 0. LDMP and RDMP counts were relatively stable over 14 days. Significant differences among filtered and nonfiltered products did not emerge.
Although the conditions of this study showed no favorable or unfavorable effects of three different filters on microparticle formation, surveillance and investigation of unanticipated outcomes in other experimental and clinical circumstances should continue.
血液成分中的微粒可能导致输血相关免疫调节或其他副作用。为了阐明白细胞滤除的作用，我们比较了三种市售的过滤器对其在单采血小板中血小板衍生的（PDMP）、白细胞衍生的（LDMP）和红细胞衍生的（RDMP）微粒形成的影响。
从一对 ABO 相同的男性供体中采集的单采血小板被汇集并分成四等份。一份不经过滤储存，而另外三份用不同的设备过滤。在 22°C 搅拌储存的 2 周内，通过流式细胞术测量 PDMPs、LDMPs 和 RDMPs。
平均而言，PDMPs 在 5 天的储存期间增加了一倍，随后增加得更快，到第 14 天 PDMPs 几乎是第 0 天的 20 倍。LDMP 和 RDMP 的计数在 14 天内相对稳定。过滤和未过滤产品之间没有出现显著差异。
尽管本研究条件显示三种不同的过滤器对微粒形成没有有利或不利的影响，但应继续监测和调查其他实验和临床情况下的意外结果。
