Gao Ming, Zhang Bin, Zhang Yue, Chen Yuting, Xiong Jin, Wang Juan, Chen Hanwei, Chen Guoan, Wei Qing
Department of Transfusion, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Nanchang University, Nanchang, Jiangxi, China.
Transfusion. 2018 Oct;58(10):2388-2394. doi: 10.1111/trf.14890. Epub 2018 Sep 10.
Although platelet microparticles (PMPs) were the most abundant micoparticles (MPs) in platelet (PLT) products, other MPs and their parental cells can also be brought into the plasma during PLT apheresis. However, the effects of PLT apheresis, storage time, and leukofiltration on these MPs remain largely unclear.
Apheresis PLTs with or without leukofiltration were stored in 22 ± 2 °C for 5 days. PLT-poor plasma (PPP) was generated by centrifugation of donor blood or PLT products at 2500 × g for 15 minutes on the point day. PPP was labeled with CD41a (PLT-derived MP, PMP), CD235 (red blood cell-derived MP, RMP), CD45 (leukocyte-derived MP, LMP), CD14 (monocyte-derived MP, MMP), and CD144 (endothelial cell-derived MP, EMP), and then measured by flow cytometry.
Higher-level TMPs (total microparticles) and PMPs, but lower-level RMPs, LMPs, and MMPs were detected in fresh PLTs on the day of collection compared with those before collection. During storage, TMP, PMP, and RMP counts were significantly higher on Day 3 and Day 5, but MMP and LMP counts were only marginally higher on Day 3 in PLT supernatants. There were no significant differences in MP levels in PLTs with or without leukofiltration.
MP formation was affected by the apheresis procedure. RMPs, LMPs and MMPs were lower after PLT apheresis. During storage, TMPs, PMPs, RMPs, LMPs, and MMPs were found to be higher in PLT supernatants. Leukofiltration exerted no significant effect on all MPs in PLT products.
尽管血小板微粒（PMPs）是血小板（PLT）制品中含量最丰富的微粒（MPs），但在血小板单采过程中，其他微粒及其来源细胞也会进入血浆。然而，血小板单采、储存时间和白细胞过滤对这些微粒的影响仍不清楚。
对经过或未经过白细胞过滤的单采血小板在22±2℃下储存5天。在采集当天，通过将供体血液或血小板制品以2500×g离心15分钟制备少血小板血浆（PPP）。用CD41a（血小板来源的微粒，PMP）、CD235（红细胞来源的微粒，RMP）、CD45（白细胞来源的微粒，LMP）、CD14（单核细胞来源的微粒，MMP）和CD144（内皮细胞来源的微粒，EMP）对PPP进行标记，然后通过流式细胞术进行检测。
与采集前相比，采集当天新鲜血小板中检测到更高水平的总微粒（TMPs）和PMPs，但RMPs、LMPs和MMPs水平较低。在储存期间，血小板上清液中TMP、PMP和RMP计数在第3天和第5天显著升高，但MMP和LMP计数仅在第3天略有升高。经过白细胞过滤和未经过白细胞过滤的血小板中微粒水平无显著差异。
微粒的形成受单采过程影响。血小板单采后RMPs、LMPs和MMPs水平较低。在储存期间，血小板上清液中TMPs、PMPs、RMPs、LMPs和MMPs水平升高。白细胞过滤对血小板制品中的所有微粒均无显著影响。
