Department of Civil and Environmental Engineering, KAIST, 373-1, Guseong-Dong, Yuseong-gu, Daejeon, Republic of Korea.
Bioresour Technol. 2013 Mar;131:210-7. doi: 10.1016/j.biortech.2012.12.100. Epub 2012 Dec 29.
The performance of dry anaerobic digestion (AD) of food waste was investigated under mesophilic conditions and the methanogenic community was investigated using 454 pyrosequencing. Stable dry AD was achieved by hydraulic retention time (HRT) control without the addition of alkali agents. The average CH4 production rate, CH4 content, and volatile solid reduction rate were 2.51±0.17m(3)/m(3)/d, 66±2.1%, and 65.8±1.22%, respectively, at an HRT of 40d. The methanogenic community of the seed sludge experienced a significant reduction in genus diversity from 18 to 4 and a dominant methanogenic shift from hydrogenotrophic to acetoclastic groups after the acclimation under dry condition. Almost all sequences of the dry anaerobic digester were closely related with those of Methanosarcina thermophila with similarity of 96.4-99.1%. The experimental results would serve as useful information to understand the dry AD system.
采用 454 焦磷酸测序技术研究了中温条件下食物垃圾干法厌氧消化（AD）的性能，并研究了产甲烷菌群。通过水力停留时间（HRT）控制，无需添加碱剂即可实现稳定的干法 AD。在 HRT 为 40d 时，平均 CH4 产率、CH4 含量和挥发性固体去除率分别为 2.51±0.17m(3)/m(3)/d、66±2.1%和 65.8±1.22%。种泥的产甲烷菌群在干燥条件下驯化后，从 18 个属减少到 4 个属，且从氢营养型向乙酸营养型的优势产甲烷菌发生了显著转变。干燥厌氧消化器中的几乎所有序列与嗜热甲烷八叠球菌（Methanosarcina thermophila）的序列密切相关，相似度为 96.4-99.1%。实验结果将为理解干法 AD 系统提供有用信息。
