Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, Ags., C. P. 20131, Mexico.
Groupe de recherche sur la maladies infectieuses du porc, Faculté de médecine vétérinaire, Université de Montréal, St-Hyacinthe, QC J2S 7C6, Canada.
Microbiology (Reading). 2013 Mar;159(Pt 3):536-544. doi: 10.1099/mic.0.057992-0. Epub 2013 Jan 24.
Actinobacillus pleuropneumoniae is the aetiological agent of porcine pleuropneumonia and is normally transmitted by aerosols and direct contact between animals. A. pleuropneumoniae has traditionally been considered an obligate pathogen of pigs and its presence in the environment has yet to be investigated. Here, the presence of A. pleuropneumoniae was detected in drinking water of pig farms in Mexico using a PCR specific for the RTX toxin gene, apxIV. The presence of A. pleuropneumoniae in farm drinking water was confirmed by indirect immunofluorescence using an A. pleuropneumoniae-specific polyclonal antibody and by fluorescent in situ hybridization. Viable bacteria from the farm drinking water were detected using the Live/Dead BacLight stain. Additionally, viable A. pleuropneumoniae was selected and isolated using the cAMP test and the identity of the isolated bacteria were confirmed by Gram staining, a specific polyclonal antibody and an A. pleuropneumoniae-specific PCR. Furthermore, biofilms were observed by scanning electron microscopy in A. pleuropneumoniae-positive samples. In conclusion, our data suggest that viable A. pleuropneumoniae is present in the drinking water of swine farms and may use biofilm as a strategy to survive in the environment.
胸膜肺炎放线杆菌是猪传染性胸膜肺炎的病原体，通常通过气溶胶和动物之间的直接接触传播。胸膜肺炎放线杆菌传统上被认为是猪的专性病原体，其在环境中的存在尚未得到研究。在这里，使用针对 RTX 毒素基因 apxIV 的 PCR 检测到了来自墨西哥养猪场饮用水中的胸膜肺炎放线杆菌。通过使用胸膜肺炎放线杆菌特异性多克隆抗体进行间接免疫荧光和荧光原位杂交，证实了农场饮用水中存在胸膜肺炎放线杆菌。使用 Live/Dead BacLight 染色检测来自农场饮用水的活菌。此外，使用 cAMP 试验选择和分离了有活力的胸膜肺炎放线杆菌，并通过革兰氏染色、特异性多克隆抗体和胸膜肺炎放线杆菌特异性 PCR 确认了分离细菌的身份。此外，在胸膜肺炎放线杆菌阳性样本中通过扫描电子显微镜观察到了生物膜。总之，我们的数据表明，有活力的胸膜肺炎放线杆菌存在于养猪场的饮用水中，可能利用生物膜作为在环境中生存的策略。
