Diabetes, Obesity, and Human Reproduction Research Group, Hospital Universitario Ramón y Cajal and Universidad de Alcalá & Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
J Clin Endocrinol Metab. 2013 Mar;98(3):E576-85. doi: 10.1210/jc.2012-3438. Epub 2013 Jan 24.
Sex hormones, particularly androgens, may influence not only adipose tissue distribution but also its functions.
We aimed to evaluate if sexual dimorphism in body composition is accompanied by differences in the protein abundance of adipose tissue by applying a nontargeted proteomic approach.
This was a case-control study.
The setting was an academic hospital.
Twenty-one morbidly obese patients, including 7 men, 7 women showing no evidence of androgen excess, and 7 hyperandrogenic women with polycystic ovary syndrome.
We obtained subcutaneous (SAT) and visceral (VAT) adipose tissue samples during bariatric surgery.
Protein abundance in VAT and SAT was analyzed by 2-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight coupled to mass spectrometry. Results were validated by RT-PCR.
The abundance of 2 spots of peroxiredoxin 6, creatine kinase B-type, 2 spots of selenium-binding protein 1, ruvB-like 2, 4-trimethylaminobutyraldehyde dehydrogenase, and albumin were higher in VAT compared with SAT in women with polycystic ovary syndrome. Men showed a similar pattern, whereas no difference between adipose tissue depots was observed in control women. Other proteins showed differences between VAT and SAT, confirming previous studies, or between the groups of subjects, without interaction between both effects. Several findings were confirmed by RT-PCR.
Sexual dimorphism influences the abundance of several proteins in VAT and SAT. The patterns of abundance in adipose tissue depots of several proteins involved in metabolic processes were similar in women with androgen excess and in men, suggesting that androgens influence adipose tissue function.
性激素，尤其是雄激素，不仅可能影响脂肪组织的分布，还可能影响其功能。
我们旨在通过应用非靶向蛋白质组学方法评估身体成分的性别二态性是否伴有脂肪组织蛋白丰度的差异。
这是一项病例对照研究。
该研究地点为一家学术医院。
21 例病态肥胖患者，包括 7 名男性、7 名无雄激素过多证据的女性和 7 名患有多囊卵巢综合征的高雄激素血症女性。
我们在减肥手术期间获得了皮下（SAT）和内脏（VAT）脂肪组织样本。
通过二维差异凝胶电泳和基质辅助激光解吸/电离-飞行时间/飞行时间与质谱联用分析 VAT 和 SAT 中的蛋白质丰度。通过 RT-PCR 验证结果。
在多囊卵巢综合征女性中，过氧化物还原酶 6、肌酸激酶 B 型、硒结合蛋白 1、ruvB 样 2、4-三甲氨基丁醛脱氢酶和白蛋白的 2 个斑点的丰度在 VAT 中高于 SAT。男性表现出相似的模式，而在对照组女性中，脂肪组织库之间没有差异。其他蛋白质在 VAT 和 SAT 之间存在差异，这证实了之前的研究，或者在研究对象组之间存在差异，两种效应之间没有相互作用。通过 RT-PCR 证实了几个发现。
性别二态性影响 VAT 和 SAT 中几种蛋白质的丰度。在雄激素过多的女性和男性中，几种参与代谢过程的蛋白质在脂肪组织库中的丰度模式相似，这表明雄激素影响脂肪组织功能。
