Diabetes, Obesity and Human Reproduction Group, Universidad de Alcalá & Hospital Universitario Ramón y Cajal & Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain.
Mol Cell Endocrinol. 2012 Nov 5;363(1-2):10-9. doi: 10.1016/j.mce.2012.07.001. Epub 2012 Jul 14.
Subcutaneous (SAT) and visceral adipose tissue (VAT) differ in biochemical and metabolic properties, especially when obesity is present. We submitted paired SAT and VAT samples from six morbidly obese patients and six non-obese persons to two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry. Compared with non-obese subjects, obese patients presented with increased carboxylesterase-1, zinc finger protein 324A, annexin A5, ubiquitin carboxyl-terminal hydrolase, α-crystallin B chain, osteoglycin, retinal dehydrogenase-1 and 14-3-3 protein γ, and decreased transferrin, complement C3, fibrinogen γ chain, albumin, α1-antitrypsin and peroxiredoxin-6, irrespective of the adipose tissue depot studied. SAT and VAT differed in protein species of fibrinogen and osteoglycin, whereas adipose tissue depot and obesity interacted on the protein abundance of actin, α-actinin 1, one protein species of carboxylesterase-1, retinal dehydrogenase-1 and 14-3-3 protein γ. Our nontargeted proteomic approach identified novel protein species that may be involved in the development of obesity in humans.
皮下（SAT）和内脏脂肪组织（VAT）在生化和代谢特性上存在差异，尤其是在肥胖存在的情况下。我们从六名病态肥胖患者和六名非肥胖者中提交了配对的 SAT 和 VAT 样本，进行二维差异凝胶电泳和基质辅助激光解吸/电离-飞行时间/飞行时间质谱分析。与非肥胖者相比，肥胖患者的羧基酯酶-1、锌指蛋白 324A、膜联蛋白 A5、泛素羧基末端水解酶、α-晶体蛋白 B 链、骨桥蛋白、视网膜脱氢酶-1 和 14-3-3 蛋白 γ 增加，转铁蛋白、补体 C3、纤维蛋白原 γ 链、白蛋白、α1-抗胰蛋白酶和过氧化物酶 6 减少，无论研究的脂肪组织库如何。SAT 和 VAT 在纤维蛋白原和骨桥蛋白的蛋白质种类上存在差异，而脂肪组织库和肥胖相互作用于肌动蛋白、α-辅肌动蛋白 1、一种羧基酯酶-1、视网膜脱氢酶-1 和 14-3-3 蛋白 γ 的蛋白质丰度上。我们的非靶向蛋白质组学方法鉴定出了可能参与人类肥胖发展的新型蛋白质种类。
