Forrest M, Isaac-Renton J, Bowie W
Division of Medical Microbiology, University of British Columbia, Vancouver, Canada.
Can J Microbiol. 1990 Jan;36(1):42-6. doi: 10.1139/m90-007.
Eighteen isolates of Giardia duodenalis from animal and human sources were studied for protein differences by polyacrylamide gel electrophoresis and for antigenic differences by immunoblot analysis. The polyacrylamide gels showed that whilst the isolates were for the most part homogeneous in their protein banding patterns, some isolates did show some differences. The immunoblot analysis yielded many bands, including prominent bands of 32 and 66 kilodaltons. Five of the six isolates that showed differences in protein banding pattern also showed differences in antigenic reactivity. Our findings suggest that differences can be seen with the use of immunoblotting and that this technique is a tool that may be useful for isolate differentiation when used in conjunction with other techniques.
对来自动物和人类的18株十二指肠贾第虫分离株进行了研究，通过聚丙烯酰胺凝胶电泳分析其蛋白质差异，并通过免疫印迹分析其抗原差异。聚丙烯酰胺凝胶显示，虽然这些分离株在蛋白质条带模式上大多是同质的，但一些分离株确实显示出一些差异。免疫印迹分析产生了许多条带，包括突出的32和66千道尔顿条带。在蛋白质条带模式上显示出差异的6株分离株中有5株在抗原反应性上也显示出差异。我们的研究结果表明，使用免疫印迹可以观察到差异，并且当与其他技术结合使用时，该技术是一种可能有助于分离株鉴别的工具。
