Wenman W M, Meuser R U, Nyugen Q, Kilani R T, el-Shewy K, Sherburne R
Department of Pediatrics, University of Alberta, Edmonton, Canada.
Parasitol Res. 1993;79(7):587-92. doi: 10.1007/BF00932243.
The trophozoites of Giardia lamblia possess several protein antigens, predominant among them a protein of approximately 32,000 Da. In the present study, we used monospecific antibodies that recognize this protein to demonstrate its presence on a variety of G. lamblia isolates from human and animal sources. Immune electron microscopy was used to localize 32-kDa antigen on the trophozoite membrane and disk. Immunofluorescent assays employing monospecific antibodies confirmed the presence of 32-kDa antigen on the membrane and disk and its absence on flagella or nuclei. The N-terminal 17 amino acids of the 32-kDa antigen are identical to alpha-1-giardin, a protein component of microribbons on the ventral disk. These results suggest that the 32-kDa immunodominant trophozoite antigen is alpha-1-giardin.
蓝氏贾第鞭毛虫的滋养体拥有多种蛋白质抗原，其中主要的一种是分子量约为32,000道尔顿的蛋白质。在本研究中，我们使用识别该蛋白质的单特异性抗体来证明其在来自人和动物源的多种蓝氏贾第鞭毛虫分离株中的存在。免疫电子显微镜用于将32 kDa抗原定位在滋养体膜和盘上。使用单特异性抗体的免疫荧光测定证实了32 kDa抗原在膜和盘上的存在以及在鞭毛或细胞核上的不存在。32 kDa抗原的N端17个氨基酸与α-1-贾第蛋白相同，α-1-贾第蛋白是腹盘微丝带上的一种蛋白质成分。这些结果表明，32 kDa免疫显性滋养体抗原是α-1-贾第蛋白。
