[猴头菇菌丝体的深层培养及化学成分]

[Submerged cultivation and chemical composition of Hericium erinaceus mycelium].

作者信息

Avtonomova A V, Bakanov A V, Shuktueva M I, Vinokurov V A, Popova O V, Usov A I, Krasnopol'skaia L M

出版信息

Antibiot Khimioter. 2012;57(7-8):7-11.

Abstract

Submerged cultivation of Hericium erinaceus in various media was studied. The yield of the biomass was shown to depend mainly on the carbon source, whereas the content of water soluble polysaccharides depended mainly on the nitrogen source. The optimal medium composition provided the biomass yield of 21-23 g/l in 7 days. The biomass was characterized by the content of total protein, lipids and carbohydrates. In addition, the amino acid composition of the biomass was determined and shown to meet all the requirements of FAO/WHO concerning the amounts of essential amino acids (with exception of tryptophane). Oleinic and linoleic acids were identified as the main components of the fatty acids. Two water soluble polysaccharide fractions differing in solubility in aqueous ethanol were isolated and shown to contain rhamnose, fucose, xylose, glucose and galactose in different proportions. Vitamins B1, B2, B6, PP and E, ergosterol and coenzyme Q were also detected in the biomass of H. erinaceus.

摘要

对猴头菌在各种培养基中的深层培养进行了研究。结果表明，生物量的产量主要取决于碳源，而水溶性多糖的含量主要取决于氮源。最佳培养基组成在7天内可提供21 - 23 g/l的生物量产量。对生物量进行了总蛋白、脂质和碳水化合物含量的表征。此外，还测定了生物量的氨基酸组成，结果表明其符合粮农组织/世界卫生组织关于必需氨基酸含量的所有要求（色氨酸除外）。油酸和亚油酸被确定为脂肪酸的主要成分。分离出了两种在乙醇水溶液中溶解度不同的水溶性多糖组分，结果表明它们含有不同比例的鼠李糖、岩藻糖、木糖、葡萄糖和半乳糖。在猴头菌生物量中还检测到了维生素B1、B2、B6、PP和E、麦角固醇和辅酶Q。