Department of Drug Technology, Faculty of Pharmacy, The Medical University of Warsaw, Warsaw, Poland.
J Ind Microbiol Biotechnol. 2009 Dec;36(12):1513-27. doi: 10.1007/s10295-009-0640-x. Epub 2009 Sep 27.
The aim of this study was to optimize the culture medium used for the mycelial growth and production of intracellular polysaccharides (IPS) and exopolysaccharides (EPS) in a submerged culture of Hericium erinaceum. Of the various factors examined, including carbon and nitrogen sources, vitamins, mineral elements, and initial pH, those that proved to have a significant effect were then tested using a 2(4) central composite rotatable design (CCRD). Under the optimal culture conditions, the maximal yield of biomass reached 14.24 +/- 0.45 g l(-1) and was 1.85-fold higher than in the basal medium. The kinetics of EPS biosynthesis in a bioreactor showed that although the highest yield of EPS (2.75 +/- 0.27 g l(-1)) could be obtained on day 8, the process of biosynthesizing high molecular weight polysaccharides proceeded until the depletion of the carbon source in the medium (after 14 days of cultivation). Our results could be very helpful in the large-scale production of bioactive polysaccharides from H. erinaceum.
本研究旨在优化长毛香菇（Hericium erinaceum）深层发酵培养的培养基，以提高菌丝体生长和胞内多糖（IPS）和胞外多糖（EPS）的产量。在考察的各种因素中，包括碳源和氮源、维生素、矿物质元素和初始 pH 值，然后使用 2(4)中心组合旋转设计（CCRD）对那些被证明有显著影响的因素进行了测试。在最佳培养条件下，生物量的最大产量达到 14.24 +/- 0.45 g l(-1)，比基础培养基提高了 1.85 倍。生物反应器中 EPS 生物合成的动力学研究表明，尽管在第 8 天可以获得最高产量的 EPS（2.75 +/- 0.27 g l(-1)），但生物合成高分子量多糖的过程一直持续到培养基中碳源耗尽（培养 14 天后）。我们的研究结果对大规模生产长毛香菇生物活性多糖具有重要意义。
