Russell M J, Vijayan V K, Gibbs R B, Geddes J W, Jacobson C H, Cotman C W
Department of Psychobiology, University of California, Irvine 92717.
Exp Neurol. 1990 May;108(2):105-8. doi: 10.1016/0014-4886(90)90015-k.
A critical issue for clinical and research applications of transplant techniques is the long-term survival of transplanted tissue and its effect on the host brain. In this study, entorhinal cortices from donor embryos were transplanted into the lesioned angular bundle of juvenile male Sprague-Dawley rats. Animals were maintained for 2 years and then sacrificed for histological and histochemical examinations. The results indicate that entorhinal transplants survive to old age and that both the host and transplant tissues maintain morphological features consistent with those of short-term neural grafts. An unexpected finding of this experiment was the persistence in the transplanted tissue and adjacent host cortex of a pattern of AChE staining which is typical of early postnatal development.
移植技术在临床和研究应用中的一个关键问题是移植组织的长期存活及其对宿主大脑的影响。在本研究中，将来自供体胚胎的内嗅皮质移植到幼年雄性Sprague-Dawley大鼠受损的角束中。动物饲养2年后处死，进行组织学和组织化学检查。结果表明，内嗅皮质移植能够存活至老年，宿主和移植组织均保持与短期神经移植相一致的形态特征。该实验的一个意外发现是，移植组织和相邻宿主皮质中持续存在一种出生后早期发育典型的乙酰胆碱酯酶染色模式。
