Gibbs R B, Harris E W, Cotman C W
J Comp Neurol. 1985 Jul 1;237(1):47-64. doi: 10.1002/cne.902370104.
The extent to which transplants of embryonic cortical tissue can be used to replace damaged cortical projections has been examined. Embryonic entorhinal cortex was implanted into the entorhinal region of young adult rats that had previously received a lesion through the angular bundle. Projections between transplant and host were examined by using WGA-HRP and the fluorescent dye Fast Blue. Implants selectively innervated areas of the host hippocampus and amygdala which normally receive entorhinal afferents. Implants were innervated by cells in the host diagonal band and, in one case, by cells in the contralateral entorhinal and/or presubicular cortex. In most cases, host fibers were differentially distributed within transplants, possibly reflecting an ability of host fibers to recognize and selectively innervate their appropriate targets even though the cellular organization of the implant is different from that present during normal development. These data suggest that homotypic implants of embryonic entorhinal cortex can, in some ways, replace severed cortical projections and may eventually be able to reconstitute normal cortical circuitry.
胚胎皮质组织移植可用于替代受损皮质投射的程度已得到研究。将胚胎内嗅皮质植入成年幼鼠的内嗅区域，这些幼鼠先前已通过角束接受了损伤。通过使用WGA-HRP和荧光染料快蓝来检查移植组织与宿主之间的投射。植入物选择性地支配宿主海马体和杏仁核中通常接受内嗅传入纤维的区域。植入物由宿主斜角带中的细胞支配，在一个案例中，还由对侧内嗅皮质和/或前下托皮质中的细胞支配。在大多数情况下，宿主纤维在移植组织内的分布存在差异，这可能反映出宿主纤维能够识别并选择性地支配其合适的靶标，即使植入物的细胞组织与正常发育期间的不同。这些数据表明，胚胎内嗅皮质的同型植入物在某些方面可以替代切断的皮质投射，并最终可能能够重建正常的皮质回路。
