Ellory J C, Hall A C, Ody S O, Englert H C, Mania D, Lang H J
Department of Physiology, University of Oxford, UK.
FEBS Lett. 1990 Mar 26;262(2):215-8. doi: 10.1016/0014-5793(90)80193-m.
Two analogues of the loop diuretics furosemide and bumetanide have been identified as differential inhibitors of KCl and NaKCl cotransport systems, assayed by measuring K+ influx in 'young' human red cells. H25 inhibited both NaKCl and KCl cotransport, with I50% values of 0.03 and 30 microM respectively; H74 had no effect on NaKCl cotransport, even at 0.3 mM, but inhibited KCl cotransport with an I50% of 75 microM. These compounds are therefore useful for resolving the two transport systems.
通过测量“年轻”人红细胞中的钾离子流入量来检测，已确定两种袢利尿剂呋塞米和布美他尼的类似物是氯化钾和钠钾氯共转运系统的差异抑制剂。H25抑制钠钾氯共转运和氯化钾共转运，其半数抑制浓度（I50%）值分别为0.03和30微摩尔；H74即使在0.3毫摩尔时对钠钾氯共转运也没有影响，但抑制氯化钾共转运的半数抑制浓度（I50%）为75微摩尔。因此，这些化合物有助于区分这两种转运系统。
