Andersson A M, Gaardsvoll H, Giladi E, Dahl B, Bock E
Research Center of Medical Biotechnology, University of Copenhagen, Denmark.
FEBS Lett. 1990 Apr 24;263(2):385-8. doi: 10.1016/0014-5793(90)81420-s.
A number of different isoforms of the neural cell adhesion molecule (NCAM) have been identified. The difference between these is due to alternative splicing of a single NCAM gene. In rat brain NCAM mRNAs with sizes of 7.4, 6.7, 5.2, 4.3 and 2.9 kb have been reported. We have synthesized six DNA oligonucleotides, that hybridize to different exons in the NCAM gene. Furthermore we have constructed three oligonucleotides, that exclusively hybridize to mRNAs lacking certain exons, by letting them consist of sequences adjacent to both sides of the splice sites. By means of these probes we have characterized the five NCAM mRNAs in rat brain.
已鉴定出神经细胞黏附分子（NCAM）的多种不同异构体。这些异构体之间的差异源于单个NCAM基因的可变剪接。据报道，在大鼠脑中存在大小为7.4、6.7、5.2、4.3和2.9 kb的NCAM mRNA。我们合成了六种与NCAM基因不同外显子杂交的DNA寡核苷酸。此外，我们通过使它们由剪接位点两侧相邻的序列组成，构建了三种仅与缺少某些外显子的mRNA杂交的寡核苷酸。借助这些探针，我们对大鼠脑中的五种NCAM mRNA进行了表征。
