使用DNA寡核苷酸探针在大鼠脑中证实与L1相关的mRNA。

Demonstration of L1-related mRNAs in rat brain using DNA oligonucleotide probes.

作者信息

Gaardsvoll H, Andersson A M, Bock E

机构信息

Research Center of Medical Biotechnology, University of Copenhagen, Panum institute, Denmark.

出版信息

FEBS Lett. 1991 Dec 2;294(1-2):94-6. doi: 10.1016/0014-5793(91)81350-h.

DOI:10.1016/0014-5793(91)81350-h

PMID:1743300

Abstract

Only one copy of the cell adhesion molecule L1 gene is present in the mouse genome, and only one mRNA of 6 kilobases (kb) is expressed in mouse brain [1987, Neurosci. Lett. 82, 89-94]. We have constructed 5 synthetic oligonucleotide probes covering different parts of the published mouse L1 cDNA sequence. Using these probes 3 distinct mRNAs of 9.0, 7.0 and 6.0 kb in rat brain could be demonstrated. Hybridizations performed at different stringency conditions indicated that the 9.0 and 7.0 kb mRNAs were highly related to the L1 mRNA of 6.0 kb expressed in rat brain. The 7.0 kb mRNA is possibly coding for a rat homologue of chicken Nr-CAM, whereas the 9.0 kb mRNA may code for a new member of the L1 family.

摘要

小鼠基因组中仅存在一份细胞粘附分子L1基因拷贝，且在小鼠脑中仅表达一种6千碱基（kb）的mRNA[1987年，《神经科学快报》82卷，89 - 94页]。我们构建了5个合成寡核苷酸探针，覆盖已发表的小鼠L1 cDNA序列的不同部分。使用这些探针，在大鼠脑中可检测到3种不同的mRNA，大小分别为9.0、7.0和6.0 kb。在不同严格条件下进行的杂交表明，9.0 kb和7.0 kb的mRNA与大鼠脑中表达的6.0 kb的L1 mRNA高度相关。7.0 kb的mRNA可能编码鸡Nr - CAM的大鼠同源物，而9.0 kb的mRNA可能编码L1家族的一个新成员。