miR-1-1 和 miR-181c 在室间隔缺损中的作用。
Roles of miR-1-1 and miR-181c in ventricular septal defects.
机构信息
Children Hospital of Fudan University, Shanghai 201102, China.
出版信息
Int J Cardiol. 2013 Sep 30;168(2):1441-6. doi: 10.1016/j.ijcard.2012.12.048. Epub 2013 Jan 24.
BACKGROUND
MicroRNAs (miRNAs) are endogenous noncoding RNAs of approximately 22 nucleotides in length that mediate post-transcriptional gene silencing by annealing to sequences in the 3'-untranslated region of target mRNAs.
METHODS
In this study, we analyzed 25 candidate miRNAs selected based on microarray data for cardiac tissue from individuals with congenital heart defects (CHDs) and from healthy control tissue.
RESULTS
This study identified specific changes in the miR-1-1 and miR-181c levels in human cardiac samples from individuals with ventricular septal defects (VSDs) relative to the levels in healthy control tissue. Increased levels of GJA1 and SOX9 were associated with the decreased expression of miR-1-1 in VSD patients, and increased miR-181c expression was correlated with downregulated BMPR2 levels. In addition, the results revealed that miR-1-1 and miR-181c directly regulate the expression of these predicted targets.
CONCLUSIONS
miR-1-1 and miR-181c are associated with the pathogenesis of VSDs.
背景
MicroRNAs (miRNAs) 是大约 22 个核苷酸长的内源性非编码 RNA，通过与靶 mRNA 的 3'非翻译区序列退火来介导转录后基因沉默。
方法
在这项研究中，我们分析了根据来自患有先天性心脏缺陷 (CHD) 的个体和健康对照组织的心脏组织的微阵列数据选择的 25 个候选 miRNAs。
结果
本研究在患有室间隔缺损 (VSD) 的个体的心脏样本中发现了 miR-1-1 和 miR-181c 水平的特定变化，与健康对照组织相比。GJA1 和 SOX9 的水平升高与 VSD 患者中 miR-1-1 的表达降低有关，而 miR-181c 的表达增加与 BMPR2 水平的下调相关。此外，结果表明 miR-1-1 和 miR-181c 直接调节这些预测靶标的表达。
结论
miR-1-1 和 miR-181c 与 VSD 的发病机制有关。
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