Zheng Chao, Dong Shuying, Tong Xuhui, Jiang Guojun, Han Xi
Department of Pharmacology, Bengbu Medical College, Bengbu, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jan;33(1):66-9.
To investigate the effect of valproate acid sodium (VPA) on gap junction intercellular communication in breast cancer Hs578T cells and explore the mechanism.
MTT assay was used to detect the cytotoxicity of VPA on Hs578T cells, and parachute assay was used to detect the effect of VPA on dye spread of the cells. Western blotting was employed to detect the expression changes of Cx43 total protein in VPA-treated Hs578T cells. The effect of VPA on the expression of Cx43 on the surface of Hs578T cells was examined with immunofluorescence assay.
MTT assay showed no obvious cytotoxicity of VPA on Hs578T cells at the concentrations below 10 mmol/L. VPA below 5 mmol/L obviously increased the gap junction function in Hs578T cells (P<0.01), and significantly enhanced the expression of Cx43 total protein (P<0.01) and Cx43 expression on the surface of Hs578T cells (P<0.01).
VPA can obviously increase the gap junction function in Hs578T cells possibly by enhancing Cx43 total protein expression and Cx43 protein expression on the surface of Hs578T cells.
研究丙戊酸钠（VPA）对乳腺癌Hs578T细胞间隙连接细胞间通讯的影响并探讨其机制。
采用MTT法检测VPA对Hs578T细胞的细胞毒性，采用降落伞试验检测VPA对细胞染料扩散的影响。运用蛋白质印迹法检测VPA处理的Hs578T细胞中Cx43总蛋白的表达变化。采用免疫荧光法检测VPA对Hs578T细胞表面Cx43表达的影响。
MTT法显示，浓度低于10 mmol/L时，VPA对Hs578T细胞无明显细胞毒性。5 mmol/L以下的VPA明显增强了Hs578T细胞的间隙连接功能（P<0.01），并显著提高了Cx43总蛋白的表达（P<0.01）以及Hs578T细胞表面Cx43的表达（P<0.01）。
VPA可能通过增强Hs578T细胞表面Cx43总蛋白表达和Cx43蛋白表达，明显提高Hs578T细胞的间隙连接功能。
