Hu Yun, He Xin, Ding Yiling
Department of Obstetrics and Gynecology, Second Xiangya Hospital, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2013 Feb;38(2):155-61. doi: 10.3969/j.issn.1672-7347.2013.02.008.
To determine the effect of human corticotrophin-releasing hormone (CRH) on the expression of connexin-43 phosphate (P-Cx43) in human myometrial smooth muscle cells (SMCs) and the function of cell gap junction intercellular communication in SMCs.
Human non-conceive myometial SMCs were cultured with different concentrations of CRH (0, 5.85, 58.5, 585 and 5850 pmol/L). Western blot was used to test P-Cx43 and Cx43 non-phosphate (NP-Cx43) of protein expression. Cell scratch was used to test cell gap junction intercellular communication opening status in human myometrial SMCs.
Compared with the control group, the expression of P-Cx43 was higher in the CRH groups (P<0.01), and was concentration-dependent. There was no significant difference in NPCx43 between the control group and the CRH groups (P>0.05). The transmission of cell layers in the CRH groups was higher than that in the control group (P<0.01), and as the concentration of CRH increased, the time was concentration-dependent (P<0.01).
CRH can enhance the expression of P-Cx43 and the function of gap junction intercellular communication in the primary cultured myometrial SMCs.
确定人促肾上腺皮质激素释放激素（CRH）对人子宫肌层平滑肌细胞（SMC）中磷酸化连接蛋白43（P-Cx43）表达及SMC中细胞间隙连接细胞间通讯功能的影响。
用不同浓度的CRH（0、5.85、58.5、585和5850 pmol/L）培养人未受孕子宫肌层SMC。采用蛋白质免疫印迹法检测P-Cx43和非磷酸化连接蛋白43（NP-Cx43）的蛋白表达。采用细胞划痕法检测人子宫肌层SMC中细胞间隙连接细胞间通讯的开放状态。
与对照组相比，CRH各处理组P-Cx43表达均升高（P<0.01），且呈浓度依赖性。对照组与CRH各处理组NPCx43比较，差异无统计学意义（P>0.05）。CRH各处理组细胞层的迁移率高于对照组（P<0.01），且随着CRH浓度增加，迁移时间呈浓度依赖性（P<0.01）。
CRH可增强原代培养子宫肌层SMC中P-Cx43的表达及间隙连接细胞间通讯功能。
