Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443 Białystok, Poland.
Environ Toxicol Pharmacol. 2013 Mar;35(2):247-53. doi: 10.1016/j.etap.2012.12.014. Epub 2013 Jan 3.
Changes in the composition and physicochemical properties of liver cell membranes due to ethanol intoxication are due mainly to reactive oxygen species (ROS). The destructive action of free radicals can be neutralized by administration of antioxidants. The purpose of this study was to investigate the efficacy of sweet grass on the physicochemical and biochemical properties of the rat liver membrane altered by chronic ethanol intoxication. Qualitative and quantitative composition of phospholipids and proteins in the membrane were determined by HPLC. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes as determined by electrophoresis. Administration of sweet grass to rats intoxicated with ethanol significantly protects lipids and proteins against oxidative modifications. Therefore, sweet grass protects against some of the deleterious membrane changes associated with ethanol exposure.
由于乙醇中毒，肝细胞膜的组成和理化性质发生变化，主要是由于活性氧（ROS）。自由基的破坏性作用可以通过给予抗氧化剂来中和。本研究的目的是探讨甜甘草对慢性乙醇中毒大鼠肝细胞膜理化性质和生化性质的影响。采用 HPLC 法测定膜中磷脂和蛋白质的定性和定量组成。乙醇增加了磷脂的水平，并改变了整体蛋白的水平，表现为苯丙氨酸、半胱氨酸和赖氨酸的减少。乙醇显著增强了电泳法测定的肝细胞膜表面电荷密度的变化。给乙醇中毒的大鼠给予甜甘草可显著保护脂质和蛋白质免受氧化修饰。因此，甜甘草可防止与乙醇暴露相关的一些有害的膜变化。
