Dobrzyńska Izabela, Szachowicz-Petelska Barbara, Ostrowska Justyna, Skrzydlewska Elzbieta, Figaszewski Zbigniew
Institute of Chemistry, University of Białystok, Al. Piłsudskiego 11/4, 15-443 Białystok, Poland.
Chem Biol Interact. 2005 Sep 10;156(1):41-53. doi: 10.1016/j.cbi.2005.07.002.
It is known that aging is characterized by changes in cell metabolism resulting in modification of the structure and function of cell membrane components which is mainly the consequence of reactive oxygen species action. These disturbances are also enhanced by different xenobiotics, e.g. ethanol. Therefore, the aim of this paper is to examine green tea influence on total antioxidant status (TAS) and on composition and electric charge of erythrocyte membrane phospholipids in ethanol intoxicated rats of various ages. Antioxidant abilities of erythrocytes were estimated by measuring TAS. Qualitative and quantitative composition of phospholipids in the membrane was determined by HPLC, while the extent of erythrocytes lipid peroxidation was estimated by HPLC measurement of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) levels. Electrophoresis was used to determine the surface charge density of the rat erythrocyte membrane. It was shown that the process of aging was accompanied by a decrease in TAS and in the total amount of phospholipids as well as by enhancement of lipid peroxidation and increase in surface charge density of erythrocyte membrane. Ethanol administration caused, in term, decrease in TAS and increase in the level of all phospholipids and lipid peroxidation products. Ethanol as well significantly enhanced changes in surface charge density of erythrocyte membrane. The ingestion of green tea partially prevented decrease in erythrocyte antioxidant abilities observed during aging and ethanol intoxication. Moreover, long-term drinking of green tea protects the structure of the erythrocytes membrane disturbed during aging process and/or chronic ethanol intoxication.
众所周知，衰老的特征是细胞代谢发生变化，导致细胞膜成分的结构和功能发生改变，这主要是活性氧作用的结果。不同的外源性物质，如乙醇，也会加剧这些干扰。因此，本文的目的是研究绿茶对不同年龄段乙醇中毒大鼠的总抗氧化状态（TAS）以及红细胞膜磷脂组成和电荷的影响。通过测量TAS来评估红细胞的抗氧化能力。用高效液相色谱法测定膜中磷脂的定性和定量组成，同时通过高效液相色谱法测量丙二醛（MDA）和4-羟基壬烯醛（4-HNE）水平来评估红细胞脂质过氧化程度。用电泳法测定大鼠红细胞膜的表面电荷密度。结果表明，衰老过程伴随着TAS和磷脂总量的减少，以及脂质过氧化的增强和红细胞膜表面电荷密度的增加。给予乙醇会导致TAS降低，所有磷脂和脂质过氧化产物水平升高。乙醇还显著增强了红细胞膜表面电荷密度的变化。摄入绿茶部分预防了衰老和乙醇中毒期间观察到的红细胞抗氧化能力的下降。此外，长期饮用绿茶可保护衰老过程和/或慢性乙醇中毒期间受到干扰的红细胞膜结构。
