Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan.
Eur J Pharmacol. 2013 Feb 28;702(1-3):12-9. doi: 10.1016/j.ejphar.2013.01.011. Epub 2013 Jan 23.
Many studies have shown that anti-inflammatory agents are effective in the treatment of endometriosis. β-Caryophyllene exerted a potent anti-inflammatory effect in vivo. However, its effect on endometriosis has not been investigated. This study aims at investigating the effect of β-caryophyllene on endometriosis and on fertility and reproduction in adult female rats. Autologous fragments of the endometrium were implantated in the peritoneal cavity in adult female rats. The growth of the endometriotic implants that developed after four weeks was recorded. Treatment started then with β-caryophyllene (10 mg/kg or 30 mg/kg) or vehicle (control) for 21 days and the growth of the endometriotic implants was measured again. In fertility studies, female rats that received β-caryophyllene or vehicle were mated and reproductive functions were observed including number and viability of implants, number of corpora lutea, length of pregnancy and outcome of litter. β-Caryophyllene (10 mg/kg) suppressed the growth of endometriotic implants by 52.5% compared with controls. Also β-caryophyllene produced apoptosis in luminal epithelim of the cyst as well as in endothelial cells of blood vessels. Ultrstructural studies revealed the presence of active mast cells and eosinophils in both control and β-caryophyllene-treated rat cysts. No statistically significant difference was observed in any studied parameter between control and β-caryophyllene-treated groups in fertility study. Therapy with β-caryophyllene may present a promising novel, non-toxic therapeutic option for patients with endometriosis.
许多研究表明，抗炎药在子宫内膜异位症的治疗中有效。β-石竹烯在体内具有强大的抗炎作用。然而，其对子宫内膜异位症的影响尚未得到研究。本研究旨在探讨β-石竹烯对子宫内膜异位症以及成年雌性大鼠生育力和生殖的影响。将子宫内膜的自体碎片植入成年雌性大鼠的腹腔中。记录四周后形成的子宫内膜异位症植入物的生长情况。然后开始用β-石竹烯（10mg/kg 或 30mg/kg）或载体（对照）治疗 21 天，并再次测量子宫内膜异位症植入物的生长情况。在生育力研究中，接受β-石竹烯或载体的雌性大鼠交配，并观察其生殖功能，包括植入物的数量和活力、黄体数量、妊娠长度和产仔情况。与对照组相比，β-石竹烯（10mg/kg）可抑制子宫内膜异位症植入物的生长，抑制率为 52.5%。β-石竹烯还可诱导囊腔的腔上皮细胞和血管内皮细胞凋亡。超微结构研究显示，在对照组和β-石竹烯处理组大鼠的囊肿中均存在活跃的肥大细胞和嗜酸性粒细胞。在生育力研究中，对照组和β-石竹烯处理组之间在任何研究参数上均无统计学差异。β-石竹烯治疗可能为子宫内膜异位症患者提供一种有前途的新型、无毒的治疗选择。
