State Key Laboratory of Hollow Fiber Membrane Materials and Processes, Key Lab of Fiber Modification & Functional Fiber of Tianjin, Tianjin Polytechnic University, Binshui West Road 399, Tianjin 300387, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Apr;106:19-24. doi: 10.1016/j.saa.2012.12.079. Epub 2013 Jan 4.
A new rhodamine-based fluorescent probe (Rh-F) for detection of Hg(2+) ions was synthesized, which could bind Hg(2+) in aqueous ethanol (7:3, v/v) at pH 7.0 with detectable change in color and fluorescence. The response is based on a ring opening reaction and formation of a 1:1 complex, while ring-opening process of spirolactam enables large fluorescent enhancement and colorimetric change upon the addition of Hg(2+). The response is reversible and linear in the range between 200nM and 1000nM, with a detection limit of 4.2nM. Selectivity and competition experiments with various other metal ion revealed that Rh-F possesses highly selective fluorescent response to Hg(2+). Furthermore, the probe was successfully applied to fluorescent imaging of Hg(2+) in L-929 cells confirm that Rh-F can be used as a fluorescent probe for monitoring Hg(2+) in living cells.
一种新的基于罗丹明的荧光探针（Rh-F）被合成用于检测 Hg(2+)离子，该探针在 pH 7.0 的水-乙醇（7:3，v/v）溶液中可以与 Hg(2+)结合，通过颜色和荧光的可检测变化来进行响应。该响应基于开环反应和 1:1 络合物的形成，而螺环酰胺的开环过程使得在加入 Hg(2+)后会产生大的荧光增强和比色变化。在 200nM 至 1000nM 范围内，响应具有可逆性和线性关系，检测限为 4.2nM。用各种其他金属离子进行的选择性和竞争实验表明，Rh-F 对 Hg(2+)具有高度选择性的荧光响应。此外，该探针成功地应用于 L-929 细胞中 Hg(2+)的荧光成像，证实 Rh-F 可以用作监测活细胞中 Hg(2+)的荧光探针。
