College of Pharmacology, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, PR China.
Anal Chim Acta. 2010 Mar 17;663(1):85-90. doi: 10.1016/j.aca.2010.01.029. Epub 2010 Jan 22.
A fluorescent probe 1 for Hg(2+) based on a rhodamine-coumarin conjugate was designed and synthesized. Probe 1 exhibits high sensitivity and selectivity for sensing Hg(2+), and about a 24-fold increase in fluorescence emission intensity is observed upon binding excess Hg(2+) in 50% water/ethanol buffered at pH 7.24. The fluorescence response to Hg(2+) is attributed to the 1:1 complex formation between probe 1 and Hg(2+), which has been utilized as the basis for the selective detection of Hg(2+). Besides, probe 1 was also found to show a reversible dual chromo- and fluorogenic response toward Hg(2+) likely due to the chelation-induced ring opening of rhodamine spirolactam. The analytical performance characteristics of the proposed Hg(2+)-sensitive probe were investigated. The linear response range covers a concentration range of Hg(2+) from 8.0x10(-8) to 1.0x10(-5)molL(-1) and the detection limit is 4.0x10(-8)molL(-1). The determination of Hg(2+) in both tap and river water samples displays satisfactory results.
一种基于罗丹明-香豆素缀合物的用于 Hg(2+) 的荧光探针 1 被设计和合成。探针 1 对 Hg(2+) 的传感表现出高灵敏度和选择性，并且在 pH 值为 7.24 的 50%水/乙醇缓冲液中与过量 Hg(2+) 结合时，荧光发射强度增加约 24 倍。对 Hg(2+) 的荧光响应归因于探针 1 与 Hg(2+) 之间的 1:1 络合形成，这已被用作选择性检测 Hg(2+) 的基础。此外，探针 1 还被发现对 Hg(2+) 表现出可逆的双重显色和荧光响应，这可能归因于罗丹明螺环酰胺的螯合诱导开环。对所提出的 Hg(2+) 敏感探针的分析性能特征进行了研究。线性响应范围涵盖了从 8.0x10(-8) 到 1.0x10(-5)molL(-1)的 Hg(2+)浓度范围，检测限为 4.0x10(-8)molL(-1)。在自来水和河水样品中测定 Hg(2+) 显示出令人满意的结果。
