Department of Chemistry, Jadavpur University, Kolkata, India.
Org Biomol Chem. 2013 Mar 7;11(9):1563-9. doi: 10.1039/c3ob27405e.
A 7-nitrobenz-2-oxa-1,3-diazole (NBD) derived turn-on fluorescent probe (1) exhibits a reversible binding to Cu(2+) ion in presence of other metal ions, giving ~20 fold increase in fluorescence intensity. The apparent association constant (K(a)) for Cu(2+) was found to be 2.62 × 10(4) M(-1). The intracellular Cu(2+) imaging behaviour of chemosensor 1 on HeLa cells studied by fluorescence microscopy revealed that after incubation with 1 cells exhibited intensive fluorescence when exogenous Cu(2+) was introduced into the cell. Thus this probe features the ready availability, high sensitivity and selectivity towards Cu(2+) in MeCN-H(2)O (1:1, v/v) with cell imaging possibilities with no or negligible cytotoxicity.
一种基于 7-硝基苯并-2-氧杂-1,3-二唑（NBD）的荧光探针（1）能够在其他金属离子存在的情况下，与 Cu(2+) 离子发生可逆结合，荧光强度增强约 20 倍。实验发现，探针（1）与 Cu(2+) 的表观结合常数（K(a)）为 2.62 × 10(4) M(-1)。通过荧光显微镜研究探针（1）在 HeLa 细胞内对 Cu(2+)的成像行为发现，当向细胞内引入外源性 Cu(2+)时，用探针（1）孵育后的细胞显示出强烈的荧光。因此，该探针具有高灵敏度、高选择性，并且在 MeCN-H(2)O（1:1，v/v）中易于获得，对 Cu(2+)的选择性好，同时具有细胞成像能力且细胞毒性低或可忽略不计。
