基于芘的高选择性铜（II）离子比率型荧光探针及其在活细胞成像中的应用。
A pyrene-based highly selective turn-on fluorescent sensor for copper(II) ion and its application in live cell imaging.
机构信息
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, 300, Republic of China.
出版信息
J Fluoresc. 2012 Jan;22(1):253-9. doi: 10.1007/s10895-011-0955-7. Epub 2011 Aug 26.
A new pyrene derivative (chemosensor 1) containing a picolinohydrazide moiety exhibits high selectivity for Cu(2+) ion detection in mixed aqueous media (CH(3)OH:H(2)O = 7:3). Significant fluorescence enhancement was observed with chemosensor 1 in the presence of Cu(2+). However, the metal ions Ag(+), Ca(2+), Cd(2+), Co(2+), Cu(2+), Fe(2+), Fe(3+), Hg(2+), K(+), Mg(2+), Mn(2+), Ni(2+), Pb(2+), and Zn(2+) produced only minor changes in fluorescence for the system. The apparent association constant (K(a)) of Cu(2+) binding in chemosensor 1 was found to be 2.75*10(3) M(-1). The maximum fluorescence enhancement caused by Cu(2+) binding in chemosensor 1 was observed over the pH range 5-8. Moreover, by means of fluorescence microscopy experiments, it is demonstrated that 1 can be used as a fluorescent probe for detecting Cu(2+) in living cells.
一种含有吡啶甲酰肼部分的新型芘衍生物（化学传感器 1）在混合水介质（CH(3)OH:H(2)O = 7:3）中对 Cu(2+)离子检测表现出高选择性。在存在 Cu(2+)的情况下，化学传感器 1 观察到显著的荧光增强。然而，金属离子 Ag(+)、Ca(2+)、Cd(2+)、Co(2+)、Cu(2+)、Fe(2+)、Fe(3+)、Hg(2+)、K(+)、Mg(2+)、Mn(2+)、Ni(2+)、Pb(2+)和 Zn(2+)仅对该体系的荧光产生微小变化。在化学传感器 1 中 Cu(2+)结合的表观结合常数（K(a)）被发现为 2.75*10(3) M(-1)。通过 Cu(2+)结合在化学传感器 1 中引起的最大荧光增强在 pH 值 5-8 的范围内观察到。此外，通过荧光显微镜实验证明，1 可以用作检测活细胞中 Cu(2+)的荧光探针。
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