Department of Anatomy, Pathologic Anatomy and Histology, Faculty of Medicine, University of La Laguna, Tenerife, Spain.
Histol Histopathol. 2013 Jul;28(7):925-32. doi: 10.14670/HH-28.925. Epub 2013 Jan 28.
The p73 proteins are present in different kinds of cells of the central nervous system, such as the choroid plexus, circumventricular structures and neuroepithelium. It has been reported that spontaneously hypertensive rats show ventricular dilation, changes in cerebrospinal fluid proteins and variations in the circumventricular structures such as the organum vasculosum of the lamina terminalis and the choroid plexus, which are altered in ventricular dilation. The aim of the present work is to study p73 expression in the organum vasculosum of the lamina terminalis and the choroid plexus and its variations in high blood pressure. Brains from control Wistar-Kyoto rats and spontaneously hypertensive rats were used. The organum vasculosum of the lamina terminalis and the choroid plexus were processed by immunohistochemistry and western blot with anti-TAp73. We found weaker markings in the organum vasculosum of the lamina terminalis and stronger markings in the choroid plexus of the hypertensive than the control rats. Therefore, hypertension in the spontaneously hypertensive rats produces alterations in choroid plexus protein p73 expression that is similar to that described for other circumventricular organs, but it is different in the organum vasculosum of the lamina terminalis. We can conclude that the functional balance between p73, organum vasculosum of the lamina terminalis and choroid plexus, which is probably necessary to maintain the normal functioning of these structures, is altered by the hypertension found in these rats.
p73 蛋白存在于中枢神经系统的不同细胞中，如脉络丛、室周结构和神经上皮。有报道称，自发性高血压大鼠表现出心室扩张、脑脊液蛋白变化以及室周结构的变化，如终板血管器官和脉络丛，这些结构在心室扩张时发生改变。本研究旨在研究 p73 在终板血管器官和脉络丛中的表达及其在高血压中的变化。使用了来自对照 Wistar-Kyoto 大鼠和自发性高血压大鼠的大脑。通过免疫组织化学和 Western blot 用抗 TAp73 处理终板血管器官和脉络丛。我们发现高血压大鼠的终板血管器官中的标记较弱，而脉络丛中的标记较强。因此，自发性高血压大鼠的高血压导致脉络丛蛋白 p73 表达发生改变，这与其他室周器官描述的改变相似，但在终板血管器官中则不同。我们可以得出结论，p73、终板血管器官和脉络丛之间的功能平衡可能对这些结构的正常功能至关重要，而这些大鼠中存在的高血压会改变这种平衡。
