免疫球蛋白 E 介导的福可定过敏和对神经肌肉阻滞剂的交叉反应：流式细胞术的教训。
IgE-mediated allergy to pholcodine and cross-reactivity to neuromuscular blocking agents: Lessons from flow cytometry.
机构信息
Department of Immunology, Allergology and Rheumatology, University Antwerp and Antwerp University Hospital, Antwerpen, Belgium.
出版信息
Cytometry B Clin Cytom. 2013 Mar;84(2):65-70. doi: 10.1002/cyto.b.21074. Epub 2013 Jan 25.
BACKGROUND
Immunoglubulin E antibody-mediated allergic reactions to opioids are rare and difficult to document correctly.
OBJECTIVE
Assessment of the basophil activation test in the diagnosis of IgE-mediated allergy to the antitussive pholcodine and associated sensitizations to neuromuscular blocking agents (NMBA).
METHODS
Three patients with a suspected IgE-mediated allergy to pholcodine were investigated using skin tests, quantification of specific IgE, and flow cytometric activation of basophils.
RESULTS AND CONCLUSION
Flow cytometric activation of basophils, with simultaneous analysis of CD63 appearance and median histamine content per cell, is the only technique capable to correctly document pholcodine allergy. The negative predictive value of basophil activation tests might help to elucidate on the controversial putative cross-reactivity between pholcodine and NMBA.
背景
免疫球蛋白 E 抗体介导的阿片类药物过敏反应很少见，且难以正确记录。
目的
评估嗜碱性粒细胞激活试验在诊断镇咳药福可定的 IgE 介导过敏反应以及对神经肌肉阻滞剂（NMBA）的相关致敏中的作用。
方法
使用皮肤试验、特异性 IgE 定量和嗜碱性粒细胞的流式细胞术激活对 3 例疑似福可定 IgE 介导过敏的患者进行了研究。
结果与结论
通过同时分析细胞内 CD63 的出现和中位组胺含量，流式细胞术激活嗜碱性粒细胞是唯一能够正确记录福可定过敏的技术。嗜碱性粒细胞激活试验的阴性预测值可能有助于阐明福可定和 NMBA 之间存在争议的假定交叉反应性。
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