School of Marine Science, Ningbo University, Ningbo 315211, PR China.
The Key Laboratory of Biomedical Information Engineering, Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, PR China.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1197-1209. doi: 10.1099/ijs.0.049403-0. Epub 2013 Jan 25.
This paper investigates the morphology, infraciliature and small-subunit (SSU) rRNA gene sequences of two hypotrichous ciliates, Neourostylopsis orientalis n. sp., and Protogastrostyla sterkii (Wallengren, 1900) n. comb. (basionym Gastrostyla sterkii), collected from coastal waters in southern China. Neourostylopsis orientalis n. sp. is diagnosed mainly by the arrangement of brownish cortical granules, the numbers of adoral membranelles and frontal and transverse cirri and the characteristics of its midventral cirral pairs. The SSU rRNA gene phylogeny strongly supports the establishment of the new genus Neourostylopsis n. gen., which is characterized mainly by the following features: frontal and transverse cirri clearly differentiated, buccal cirri present, two frontoterminal cirri, midventral complex composed of midventral pairs only and not exceeding the halfway point of the cell, more than one row of marginal cirri on each side which derive from individual anlagen within each parental row, caudal cirri lacking. Thus, two new combinations are required: Neourostylopsis songi (Lei et al., 2005) n. comb., and Neourostylopsis flavicana (Wang et al., 2011) n. comb. Additionally, improved diagnoses for both Metaurostylopsis and Apourostylopsis are supplied in this study. Protogastrostyla sterkii (Wallengren, 1900) n. comb. differs from the similar congener Protogastrostyla pulchra mainly in body shape, ratio of buccal field to body length in vivo and molecular data. Based on the present studies, we conclude that the estuarine population of P. pulchra collected by J. Gong and others [Gong et al., J Eukaryot Microbiol (2007) 54, 468-478] is a population of P. sterkii.
本文研究了采自中国南方沿海海域的两种缘毛目纤毛虫，东方新毛口虫（Neourostylopsis orientalis n. sp.）和斯特氏前口盘虫（Protogastrostyla sterkii）（合胞体 Gastrostyla sterkii）的形态、纤毛图式和小亚基（SSU） rRNA 基因序列。东方新毛口虫主要通过棕色皮质颗粒的排列、口围纤毛器的数目、额和横棘毛以及中腹侧纤毛列的特征来诊断。SSU rRNA 基因系统发育强烈支持建立新属 Neourostylopsis n. gen.，其主要特征如下：额和横棘毛明显分化，具口纤毛，有 2 根前侧棘毛，中腹复杂结构仅由中腹侧对组成，不超过细胞的中点，每侧有超过一排的缘毛，由每个亲代排内的个别原基衍生而来，无尾棘毛。因此，需要两个新的组合：Neourostylopsis songi（Lei 等人，2005）n. comb.和 Neourostylopsis flavicana（Wang 等人，2011）n. comb.此外，本研究还提供了对 Metaurostylopsis 和 Apourostylopsis 的改进诊断。Protogastrostyla sterkii（Wallengren，1900）n. comb.与相似的同属种 Protogastrostyla pulchra 主要在体形、活体口域与体长的比例以及分子数据上有所不同。基于目前的研究，我们得出结论，由 J. Gong 等人采集的河口种群 Protogastrostyla pulchra（Gong 等人，J Eukaryot Microbiol（2007）54，468-478）实际上是 P. sterkii 的一个种群。
