Elkins T, Hortsch M, Bieber A J, Snow P M, Goodman C S
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
J Cell Biol. 1990 May;110(5):1825-32. doi: 10.1083/jcb.110.5.1825.
Fasciclin I is a membrane-associated glycoprotein that is regionally expressed on a subset of fasciculating axons during neuronal development in insects; it is expressed on apposing cell surfaces, suggesting a role in specific cell adhesion. In this paper we show that Drosophila fasciclin I is a novel homophilic cell adhesion molecule. When the nonadhesive Drosophila S2 cells are transfected with the fasciclin I cDNA, they form aggregates that are blocked by antisera against fasciclin I. When cells expressing fasciclin I are mixed with cells expressing fasciclin III, another Drosophila homophilic adhesion molecule, the mixture sorts into aggregates homogeneous for either fasciclin I- or fasciclin III-expressing cells. The ability of these two novel adhesion molecules to mediate cell sorting in vitro suggests that they might play a similar role during neuronal development.
成束蛋白I是一种与膜相关的糖蛋白，在昆虫神经元发育过程中，它在成束轴突的一个亚群上区域表达；它在相对的细胞表面表达，表明其在特定细胞黏附中起作用。在本文中，我们表明果蝇成束蛋白I是一种新型的同源细胞黏附分子。当用成束蛋白I cDNA转染无黏附性的果蝇S2细胞时，它们会形成聚集体，而这些聚集体会被抗成束蛋白I的抗血清阻断。当表达成束蛋白I的细胞与表达成束蛋白III（另一种果蝇同源黏附分子）的细胞混合时，混合物会分选成表达成束蛋白I或成束蛋白III的细胞的同质聚集体。这两种新型黏附分子在体外介导细胞分选的能力表明，它们可能在神经元发育过程中发挥类似作用。
