Department of Pathology, Keio University, Tokyo, Japan.
Pathol Int. 2013 Jan;63(1):13-9. doi: 10.1111/pin.12013. Epub 2012 Dec 16.
Gastric cancer is one of the most common malignancies worldwide and patients with advanced gastric cancer still have poor clinical outcomes. The overexpression of leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5) mRNA in colorectal cancer and its correlation with clinicopathological factors were recently reported by us. In this study, we show LGR5 mRNA overexpression in human gastric cancer specimens by quantitative RT-PCR and in situ hybridization and assess a correlation with clinicopathological factors. The mean expression of LGR5 mRNA in cancerous tissues was five times higher than that in normal tissue (P = 0.0002). Furthermore, LGR5 mRNA expression show marked variation among cases and significantly increased in cases where lymphatic invasion was present compared with those where it was absent (P = 0.0056). Although the mean expression level of LGR5 was observed to be higher in nodal metastasis and venous invasion positive cases compared to negative cases, a significant difference was not observed. These results suggest that LGR5 can be a biomarker for malignancy in gastric cancer.
胃癌是全球最常见的恶性肿瘤之一，晚期胃癌患者的临床预后仍然较差。我们最近报道了富含亮氨酸重复的 G 蛋白偶联受体 5（LGR5）mRNA 在结直肠癌中的过表达及其与临床病理因素的相关性。在这项研究中，我们通过定量 RT-PCR 和原位杂交显示了人胃癌标本中 LGR5 mRNA 的过表达，并评估了其与临床病理因素的相关性。癌组织中 LGR5 mRNA 的平均表达量是正常组织的五倍（P=0.0002）。此外，LGR5 mRNA 的表达在病例之间存在明显差异，与无淋巴浸润的病例相比，存在淋巴浸润的病例表达明显增加（P=0.0056）。尽管与阴性病例相比，淋巴结转移和静脉浸润阳性病例的 LGR5 平均表达水平更高，但未观察到显著差异。这些结果表明，LGR5 可以作为胃癌恶性程度的生物标志物。
