National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Perth, Western Australia 6009, Australia.
Lung Cancer. 2013 Apr;80(1):39-44. doi: 10.1016/j.lungcan.2012.12.008. Epub 2013 Jan 26.
The biomarker mesothelin is a useful diagnostic tool in malignant mesothelioma (MM) patients. It has high specificity but a sensitivity of only 50%. As mesothelin binds CA125, and as CA125 is often elevated in MM, we asked whether this binding affected measurable mesothelin levels in a relevant clinical setting. Mesothelin and CA125 concentrations were measured in the serum of 41 patients with MM. An assay was developed to detect mesothelin bound to CA125. Mesothelin was demonstrated to be bound by CA125 in 9 of 41 MM patients. This binding could be broken by the addition of sodium dodecyl sulphate and diethylenetriaminepenta acetic acid (DTPA) to the samples, however this did not lead to any change in the mesothelin level measured. We therefore conclude that binding of mesothelin to CA125 does not alter the measurement of mesothelin for the detection of MM.
间皮素标志物是恶性间皮瘤（MM）患者有用的诊断工具。它具有很高的特异性，但敏感性只有 50%。由于间皮素与 CA125 结合，而 CA125 在 MM 中经常升高，我们想知道这种结合是否会影响相关临床环境中可测量的间皮素水平。在 41 名 MM 患者的血清中测量了间皮素和 CA125 浓度。开发了一种检测与 CA125 结合的间皮素的检测方法。在 41 名 MM 患者中有 9 名显示间皮素与 CA125 结合。通过向样品中添加十二烷基硫酸钠和二乙三胺五乙酸（DTPA）可以破坏这种结合，但这并没有导致测量的间皮素水平发生任何变化。因此，我们得出结论，间皮素与 CA125 的结合不会改变间皮素的测量，以用于检测 MM。
