Lee J D, Swisher S G, Minehart E H, McBride W H, Economou J S
Department of Surgery, UCLA School of Medicine 90024.
J Leukoc Biol. 1990 May;47(5):475-9. doi: 10.1002/jlb.47.5.475.
We report that recombinant human interleukin-4 (IL-4) downregulates interleukin-6 (IL-6) production by human peripheral blood mononuclear cells (PBMC). PBMC were preincubated for up to 24 hr in the presence of IL-4 (100 U/ml) and then activated with lipopolysaccharide B Escherichia coli 026:B6 (LPS, 10 micrograms/ml), recombinant human tumor necrosis factor-alpha (TNF-alpha, 200 U/ml), or Concanavalin A (Con A, 10 micrograms/ml). Although all these signals induced IL-6 production, IL-4-treated cells produced significantly reduced levels of IL-6 protein. This effect was dose and time dependent. We conclude that IL-4 is a potent downregulatory modulator of IL-6 expression in human PBMC.
我们报告重组人白细胞介素-4（IL-4）可下调人外周血单个核细胞（PBMC）产生白细胞介素-6（IL-6）。将PBMC在IL-4（100 U/ml）存在的情况下预孵育长达24小时，然后用大肠杆菌026:B6脂多糖（LPS，10微克/ml）、重组人肿瘤坏死因子-α（TNF-α，200 U/ml）或伴刀豆球蛋白A（Con A，10微克/ml）激活。尽管所有这些信号均诱导IL-6产生，但经IL-4处理的细胞产生的IL-6蛋白水平显著降低。这种效应呈剂量和时间依赖性。我们得出结论，IL-4是人类PBMC中IL-6表达的一种有效下调调节因子。
