Department of Environmental Science, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Colloids Surf B Biointerfaces. 2013 May 1;105:137-43. doi: 10.1016/j.colsurfb.2012.12.010. Epub 2013 Jan 3.
Quaternized chitosan (QC)-organic rectorite (OREC) intercalated composites based nanoparticles were immobilized on cellulose acetate nanofibrous mats by layer-by-layer deposition technique via hydrogen bonds. The key design of those mats was the utilization of all negatively charged materials to reduce the quantity of nanoparticles immobilized on the fibers and then the cytotoxicity of the mats. The intercalated structure in nanoparticles was confirmed by selected area electron diffraction. The morphology and composition of NPs-assembled nanofibrous mats were studied by field emission scanning electron microscopy and X-ray photoelectron spectroscopy. The results trended to give clues as how the assembly process varied by adding OREC. MTT assay and cell culture experiments showed that NPs-assembled nanofibrous mats were commendably compatible with normal cells and could selectively kill human lung carcinoma epithelial cells. Hemolysis test indicated these mats had excellent blood compatibility. As a result, QC-OREC NPs-assembled nanofibrous mats by homocharged deposition process can be considered as a novel alternative method for cancer therapy.
基于季铵化壳聚糖（QC）-有机累托石（OREC）插层复合材料的纳米粒子通过层层沉积技术通过氢键固定在醋酸纤维素纳米纤维垫上。这些垫子的关键设计是利用所有带负电荷的材料来减少固定在纤维上的纳米粒子的数量，从而降低垫子的细胞毒性。通过选区电子衍射证实了纳米粒子中的插层结构。通过场发射扫描电子显微镜和 X 射线光电子能谱研究了 NPs 组装的纳米纤维垫的形态和组成。结果表明，添加 OREC 会如何改变组装过程。MTT 测定和细胞培养实验表明，NPs 组装的纳米纤维垫与正常细胞非常兼容，并且可以选择性地杀死人肺癌上皮细胞。溶血试验表明这些垫子具有优异的血液相容性。因此，通过同种电荷沉积过程制备的 QC-OREC NPs 组装纳米纤维垫可以被认为是癌症治疗的一种新的替代方法。
