Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, USA.
IEEE Trans Ultrason Ferroelectr Freq Control. 2013 Feb;60(2):320-31. doi: 10.1109/TUFFC.2013.2568.
This paper develops a novel two-frequency approach for noninvasive evaluation of cancerous tissue with optimum depth and resolution. Frequencies of about 50 MHz are used in thickly sliced tissue to detect differences of the relative attenuation (C-scan mode scanning) with relatively limited resolution. Thus, suspect zones can be identified according to a quantitative criterion. These suspect zones are then selected for preparation of thin, transversal slices from within the original thick slices. Very-high-resolution (1-μm) visualization of cells is obtained at around 600 MHz on these transversal sections and adjacent sections are prepared for histological study in parallel. The technique's feasibility and potential are demonstrated on both normal and cancerous (melanoma) skin tissue. Isotropy of the specimens is experimentally verified to ensure that conditions were coherent for use of a 5-layer, angular spectrum model made to simulate longitudinal velocity, allowing estimation of longitudinal velocity from semiquantitative V(z) data.
本文提出了一种新颖的双频率方法，用于非侵入性评估具有最佳深度和分辨率的癌组织。在厚切片组织中使用约 50MHz 的频率来检测相对衰减的差异（C 扫描模式扫描），分辨率相对有限。因此，可以根据定量标准识别可疑区域。然后，根据这些可疑区域从原始厚切片中制备薄的横向切片。在这些切片上，大约在 600MHz 处可以获得非常高分辨率（1μm）的细胞可视化，并且相邻的切片被并行制备以进行组织学研究。该技术在正常和癌（黑色素瘤）皮肤组织上的可行性和潜力都得到了证明。实验验证了标本的各向同性，以确保使用 5 层角谱模型模拟纵向速度的条件是一致的，从而可以根据半定量 V(z)数据估计纵向速度。
