Barr R J, White G M, Jones J P, Shaw L B, Ross P A
Department of Dermatology, University of California, Irvine.
J Invest Dermatol. 1991 Jan;96(1):38-42. doi: 10.1111/1523-1747.ep12514712.
Acoustic microscopy utilizes high frequency ultrasound to generate microscopic images. The current study was designed to examine representative disorders of the skin by use of a reflective scanning acoustic microscope (R-SAM), and to determine whether the obtainable resolution was sufficient to render a microscopic diagnosis. An Olympus UH3 Scanning Acoustic Microscope was utilized with lenses producing burst wave frequencies at 600 and 800 MHz (600 and 800 million cylces/sec). Cutaneous tissue specimens representing 12 different neoplastic and inflammatory disorders were examined. Acoustic images of unstained sections were compared with conventional light microscopic study of sections stained with hematoxylin-eosin. In most neoplasms examined, it was possible to make a specific diagnosis primarily from low magnification pattern analysis. Although individual cells could be visualized, cytologic atypia was poorly defined. In the inflammatory disorders, a specific diagnosis was possible in all but bullous pemphigoid and lichen planus, because the composition of the inflammatory infiltrate was difficult to determine. The advantages of the R-SAM include the capability of producing an acoustic profile of the tissue and the future possibility of in situ diagnosis.
声学显微镜利用高频超声生成微观图像。本研究旨在使用反射扫描声学显微镜（R-SAM）检查皮肤的代表性疾病，并确定可获得的分辨率是否足以进行微观诊断。使用了一台奥林巴斯UH3扫描声学显微镜，其透镜产生频率为600和800兆赫（6亿和8亿周/秒）的脉冲波。检查了代表12种不同肿瘤性和炎症性疾病的皮肤组织标本。将未染色切片的声学图像与苏木精-伊红染色切片的传统光学显微镜研究进行了比较。在大多数检查的肿瘤中，主要通过低倍镜模式分析就有可能做出明确诊断。虽然可以看到单个细胞，但细胞异型性定义不明确。在炎症性疾病中，除大疱性类天疱疮和扁平苔藓外，在所有疾病中都有可能做出明确诊断，因为炎症浸润的成分难以确定。R-SAM的优点包括能够生成组织的声学剖面图以及未来进行原位诊断的可能性。
