镉染毒 30 天小鼠肝基因表达的 DNA 微阵列分析。

DNA microarray analysis of hepatic gene expression in mice exposed to cadmium for 30 days.

机构信息

Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University, Tokyo, Japan.

出版信息

J Toxicol Sci. 2013 Feb;38(1):155-7. doi: 10.2131/jts.38.155.

DOI:10.2131/jts.38.155

Abstract

Although cadmium causes hepatotoxicity, its molecular mechanism is unclear. In the present study, transcriptional responses in the liver of C57BL/6J mice given 50 ppm cadmium as a drinking water for 30 days were evaluated with DNA microarray. Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were not elevated following the administration of cadmium. Cadmium increased the expressions of 2 genes and reduced those of 15 genes in the liver of mice before the leading to hepatotoxicity.

摘要

虽然镉会导致肝毒性，但其中的分子机制尚不清楚。在本研究中，通过 DNA 微阵列分析评估了 50 ppm 镉作为饮用水给予 C57BL/6J 小鼠 30 天后肝脏的转录反应。血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性在给予镉后并未升高。在导致肝毒性之前，镉增加了小鼠肝脏中 2 个基因的表达，减少了 15 个基因的表达。