Beijing Ophthalmology and Visual Science Laboratory, Beijing Tongren Eye Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China.
Mol Med Rep. 2013 Mar;7(3):869-74. doi: 10.3892/mmr.2013.1294. Epub 2013 Jan 28.
The aim of the present study was to explore the survival of pig-rhesus corneal xenografts following donor bone marrow transplantation (BMT). Wuzhishan pigs were used as donors and rhesus monkeys as recipients for corneal xenotransplantation. Twelve rhesus monkeys were divided into two groups. Group 1 received intravenous injection of cyclophosphamide (CP) followed by pig bone marrow cell transplantation, while group 2 was used as a control and only received intravenous CP injection. All xenografts were evaluated using a slit-lamp microscope. The immunological status of the recipients following transplantation, including the formation of chimerism, mixed lymphocyte reaction (MLR) and immunoglobulin and complement in the serum, was analyzed. Two rhesus monkeys in each group were sacrificed for corneal histopathology examination. The mean survival time was 36.0±4.7 days in group 1 and 17.7±3.2 days in group 2. The mean chimerism percentage in group 1 at week 1 was 5.20±1.02%, but decreased with time and was <1% after week 3. MLR demonstrated that immune reactivity to donor spleen cells in group 1 was decreased following surgery. Immunoglobulin and complement levels in the serum revealed a decreasing trend. Histopathological examination demonstrated that the corneal xenografts in group 1 had minimal inflammatory cell infiltration and no eosinophil infiltration. Survival of corneal xenografts may be prolonged by prior BMT, suggesting that immune reactivity to donors is suppressed, and is highly dependent on chimerism formation.
本研究旨在探讨供体骨髓移植（BMT）后猪-恒河猴角膜异种移植物的存活情况。五指山猪作为供体，恒河猴作为角膜异种移植的受体。将 12 只恒河猴分为两组。组 1 接受环磷酰胺（CP）静脉注射，然后进行猪骨髓细胞移植，组 2 作为对照，仅接受 CP 静脉注射。所有异种移植物均通过裂隙灯显微镜进行评估。分析移植后受者的免疫状态，包括嵌合体形成、混合淋巴细胞反应（MLR）以及血清中的免疫球蛋白和补体。每组 2 只恒河猴进行角膜组织病理学检查。组 1 的平均存活时间为 36.0±4.7 天，组 2 为 17.7±3.2 天。组 1 在第 1 周的嵌合体百分比平均为 5.20±1.02%，但随时间推移而下降，第 3 周后<1%。MLR 表明组 1 手术后对供体脾细胞的免疫反应性降低。血清中免疫球蛋白和补体水平呈下降趋势。组织病理学检查显示组 1 的角膜异种移植物仅有少量炎症细胞浸润，无嗜酸性粒细胞浸润。预先进行 BMT 可延长角膜异种移植物的存活时间，表明对供者的免疫反应性受到抑制，并且高度依赖于嵌合体的形成。
