Tarakji Bassel, Baroudi Kusai, Hanouneh Salah, Nassani Mohammad Z, Alotaibi Abdullah M, Kharma M Yaser, Azzeghaiby Saleh N
Alfarabi Dental School, Kingdom of Saudi Arabia.
Pol J Pathol. 2012 Dec;63(4):286-91. doi: 10.5114/pjp.2012.32778.
Our study is aimed to characterize alteration in the immunohistochemical expression of p21 and p53 in normal tissue of the salivary gland surrounding carcinoma arising in pleomorphic adenoma, and the tumor cells of carcinoma arising in pleomorphic adenoma as well as to identify whether the induction of expression p21 is dependent on or independent of p53 in carcinoma arising in pleomorphic adenoma. A selected series of 27 cases of carcinoma ex pleomorphic adenoma (undifferentiated and adenocarcinoma types) was examined. The results showed that p21 and p53 expression was negative in the most components of normal tissue of the salivary gland surrounding carcinoma arising in pleomorphic adenoma. p21 was strongly expressed in carcinoma cells in 9 (33.3%) cases out of 27. p53 was strongly expressed in carcinoma cells in 10 (37%) cases out of 27. Also a co-expression of p21 and p53 showed negative nuclear staining in 9 cases, while 8 cases expressed positive staining. p21 expressed negative nuclear staining in 4 cases but p53 expressed positive staining in the same cases. p21 expressed positive nuclear staining in 6 cases but p53 expressed negative nuclear staining in the same cases. Our data suggest that inactivation of p53 and p21 may play an important role in the evolution of carcinoma ex pleomorphic adenoma. Also p21 behaves as dependent on or independent of p53 in carcinoma arising in pleomorphic adenoma.
我们的研究旨在描述多形性腺瘤恶变周围涎腺正常组织以及多形性腺瘤恶变肿瘤细胞中p21和p53免疫组化表达的变化，并确定多形性腺瘤恶变中p21表达的诱导是否依赖于p53或与之无关。我们选取了27例多形性腺瘤恶变（未分化型和腺癌型）病例进行研究。结果显示，多形性腺瘤恶变周围涎腺正常组织的大部分成分中p21和p53表达均为阴性。27例中有9例（33.3%）癌细胞中p21呈强表达。27例中有10例（37%）癌细胞中p53呈强表达。此外，9例p21和p53共表达显示核染色阴性，而8例呈阳性染色。4例p21核染色阴性但p53在同一病例中呈阳性染色。6例p21核染色阳性但p53在同一病例中呈阴性染色。我们的数据表明，p53和p21的失活可能在多形性腺瘤恶变的发展中起重要作用。而且在多形性腺瘤恶变中，p21的表现依赖于或不依赖于p53。
