Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, USA.
J Biomed Mater Res A. 2013 Sep;101(9):2507-14. doi: 10.1002/jbm.a.34549. Epub 2013 Jan 28.
Problems associated with current bone substitutes led to an increased interest in tissue engineered surrogates with properties similar to natural bones. Commonly used materials lack the ultimate mechanical properties. In this study, we examined the influence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) as a crosslinking agent on the strength of collagen fibers. Collagen fibers are crosslinked with EDC, or EDC/N-hydroxysulfosuccinimide (Sulfo-NHS) in water or EDC in acetone. After which fibers are mineralized using calcium chloride and potassium phosphate. The mechanical properties of the treated fibers are measured using tensile testing. In addition, the effect of crosslinking on cellular behavior was tested by culturing bone marrow mesenchymal stem cells (BMMSCs) on modified fibers. We found that the mechanical properties of non-mineralized and mineralized collagen fibers are significantly affected by the crosslinking method. The tensile strength of single fibers is greatly improved by crosslinking with EDC in acetone. BMMSCs were found to attach and spread well on modified fibers confirming biocompatibility.
当前骨替代物存在的问题促使人们对具有类似天然骨特性的组织工程替代品产生了浓厚的兴趣。常用材料缺乏最终的机械性能。在这项研究中，我们研究了 1-乙基-3-(3-二甲基氨基丙基)碳二亚胺盐酸盐（EDC）作为交联剂对胶原纤维强度的影响。EDC 将胶原纤维交联，或者在水中用 EDC/N-羟基琥珀酰亚胺（Sulfo-NHS）交联，或者在丙酮中用 EDC 交联。然后用氯化钙和磷酸钾对纤维进行矿化。使用拉伸试验测量处理后的纤维的机械性能。此外，还通过在改性纤维上培养骨髓间充质干细胞（BMMSCs）来测试交联对细胞行为的影响。我们发现，非矿化和矿化胶原纤维的机械性能受到交联方法的显著影响。在丙酮中用 EDC 交联可大大提高单纤维的拉伸强度。发现 BMMSCs 很好地附着和铺展在改性纤维上，证实了其生物相容性。
