Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education, School of Chemistry & Environment, Beihang University, Beijing, 100191, PR China.
Analyst. 2013 Mar 21;138(6):1726-32. doi: 10.1039/c2an36529d.
Oncoprotein platelet derived growth factor-BB (PDGF-BB) is one of the most critical growth factors that regulates tumor growth and division. In this work, a highly sensitive and selective fluorescence resonance energy transfer (FRET) aptasensor for PDGF-BB detection based on the assembly of dye-labeled aptamer and graphene oxide (GO) is developed for the first time. Due to the non-covalent assembly between aptamer and GO, fluorescence quenching of the dye takes place because of FRET. In the presence of PDGF-BB, the binding between aptamer and PDGF-BB will disturb the interaction between aptamer and GO, and release the dye-labeled aptamer from the GO surface, resulting in restoration of the fluorophore fluorescence. Because of the high fluorescence quenching efficiency, unique structure, and electronic properties of GO, the GO aptasensor exhibits extraordinarily high sensitivity. We also demonstrate that two highly related molecular variants of PDGF (AA, AB) can be distinguished from PDGF-BB, which indicates the aptasensor has excellent selectivity. Such an aptasensor opens a rapid, selective and sensitive route for the detection of PDGF-BB and provides a promising strategy for other cancer-related proteins detections.
癌蛋白血小板衍生生长因子-BB（PDGF-BB）是调节肿瘤生长和分裂的最关键生长因子之一。在这项工作中，首次开发了基于染料标记的适体与氧化石墨烯（GO）组装的用于 PDGF-BB 检测的高灵敏度和选择性荧光共振能量转移（FRET）适体传感器。由于适体与 GO 之间的非共价组装，染料的荧光猝灭是由于 FRET 发生的。在 PDGF-BB 存在的情况下，适体与 PDGF-BB 之间的结合会干扰适体与 GO 之间的相互作用，并将染料标记的适体从 GO 表面释放出来，从而恢复荧光团的荧光。由于 GO 具有高荧光猝灭效率、独特的结构和电子特性，GO 适体传感器表现出极高的灵敏度。我们还证明，两种高度相关的 PDGF 分子变体（AA、AB）可以与 PDGF-BB 区分开来，这表明该适体传感器具有优异的选择性。这种适体传感器为 PDGF-BB 的检测开辟了一条快速、选择性和灵敏的途径，并为其他与癌症相关的蛋白质检测提供了有前途的策略。
