State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
J Appl Microbiol. 2013 May;114(5):1480-90. doi: 10.1111/jam.12153. Epub 2013 Mar 1.
To examine the inhibition effects of rhizosphere fungal strain MF-91 on the rice blast pathogen Magnaporthe grisea and sheath blight pathogen Rhizoctonia solani.
Rhizosphere fungal strain MF-91 and its metabolites suppressed the in vitro mycelial growth of R. solani. The inhibitory effect of the metabolites was affected by incubation temperature, lighting time, initial pH and incubation time of rhizosphere fungal strain MF-91. The in vitro mycelial growth of M. grisea was insignificantly inhibited by rhizosphere fungal strain MF-91 and its metabolites. The metabolites of rhizosphere fungal strain MF-91 significantly inhibited the conidial germination and appressorium formation of M. grisea. Moreover, the metabolites reduced the disease index of rice sheath blight by 35·02% in a greenhouse and 57·81% in a field as well as reduced the disease index of rice blast by 66·07% in a field. Rhizosphere fungal strain MF-91 was identified as Chaetomium aureum based on the morphological observation, the analysis of 18S ribosomal DNA internal transcribed spacer sequence and its physiological characteristics, such as the optimal medium, temperature and initial pH for mycelial growth and sporulation production.
Rhizosphere fungus C. aureum is effective in the biocontrolling of rice blast pathogen M. grisea and sheath blight pathogen R. solani both in in vitro and in vivo conditions.
This study is the first to show that rhizosphere fungus C. aureum is a potential fungicide against rice blast and sheath blight pathogens.
研究根际真菌 MF-91 对稻瘟病菌和纹枯病菌的抑制作用。
根际真菌 MF-91 及其代谢产物抑制了 Rhizoctonia solani 的菌丝体外生长。代谢产物的抑制作用受培养温度、光照时间、初始 pH 值和根际真菌 MF-91 的培养时间的影响。根际真菌 MF-91 及其代谢产物对 M. grisea 的菌丝体外生长无显著抑制作用。根际真菌 MF-91 的代谢产物显著抑制了 M. grisea 的分生孢子萌发和附着胞形成。此外，代谢产物在温室中使稻纹枯病的病情指数降低了 35.02%，在田间降低了 57.81%，使稻瘟病的病情指数在田间降低了 66.07%。根据形态学观察、18S 核糖体 DNA 内转录间隔区序列分析以及最适培养基、温度和初始 pH 值等生理特性，将根际真菌 MF-91 鉴定为嗜热毛壳菌。
根际真菌 Chaetomium aureum 在体外和体内条件下对稻瘟病菌 M. grisea 和纹枯病菌 R. solani 的生物防治均有效。
本研究首次表明，根际真菌 Chaetomium aureum 是一种防治稻瘟病和纹枯病的潜在杀菌剂。
