State Key Laboratory of Rice Biology, Institution of Crop Science, Zhejiang University, Hangzhou, China.
Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang, China.
J Appl Microbiol. 2018 Mar;124(3):779-796. doi: 10.1111/jam.13678. Epub 2018 Jan 31.
To examine the biocontrol activities of five rhizobacterial strains (i.e. Alcaligenes faecalis strains Bk1 and P1, Bacillus amyloliquefaciens strain Bk7 and Brevibacillus laterosporus stains B4 and S5), to control the rice blast and sheath blight diseases in greenhouse and to study their possible modes of action.
Five potential plant growth-promoting rhizobacterial (PGPR) strains isolated from rice rhizospheres were tested for in vitro antifungal activities against Magnaporthe oryzae, Rhizoctonia solani, Botrytis cinerea and Fusarium graminearum. In vitro trials showed that three strains, Bk1, P1 and Bk7, were able to unanimously suppress the mycelial growth of the target pathogens. In greenhouse, the application of these three PGPR strains significantly suppressed the incidences of rice blast and sheath blight diseases. At 2 weeks after pathogen inoculation, the highest percentages of disease suppression were noted for Alc. faecalis strain Bk1 (72%) for rice blast, Alc. faecalis strain P1 (71%) for sheath blight, followed by B. amyloliquefaciens strain Bk7. Moreover, these strains significantly improved the plant growth, enriched the content of mineral nutrients in seedlings and increased the expression of major defence-related rice genes. All three strains were marked positive for phosphate solubilization, the production of indoleacetic acid, ammonia and siderophores and catalase activity. In addition, these strains were able to form biofilms and carried multiple lipopeptide biosynthetic genes as revealed by multiplex PCR.
This study reports new potential biocontrol agents for blast and sheath blight diseases of rice.
This study contributes to better understanding of the mechanisms involved in interaction between beneficial rhizobacteria, fungal pathogens and host plants.
研究 5 株根际细菌（即解淀粉芽孢杆菌 Bk1 和 P1、枯草芽孢杆菌 Bk7 和短小芽孢杆菌 B4 和 S5）的生防活性，以控制温室稻瘟病和纹枯病，并研究其可能的作用模式。
从水稻根际中分离出 5 株具有潜在植物促生作用的根际细菌（PGPR）菌株，测试其对稻瘟病菌、立枯丝核菌、灰葡萄孢和禾谷镰刀菌的体外抑菌活性。体外试验表明，3 株菌 Bk1、P1 和 Bk7 能一致抑制靶病原菌的菌丝生长。在温室中，这 3 株 PGPR 菌株的应用显著抑制了稻瘟病和纹枯病的发生。在病原菌接种后 2 周，粪肠球菌 Bk1 对稻瘟病的抑制率最高（72%），粪肠球菌 P1 对纹枯病的抑制率最高（71%），其次是解淀粉芽孢杆菌 Bk7。此外，这些菌株显著促进了植物的生长，增加了幼苗中矿质营养的含量，并提高了主要防御相关水稻基因的表达。3 株菌均对磷酸盐溶解、吲哚乙酸、氨和铁载体以及过氧化氢酶活性呈阳性。此外，这些菌株能够形成生物膜，并通过多重 PCR 携带多种脂肽生物合成基因。
本研究报道了防治水稻稻瘟病和纹枯病的新的潜在生防剂。
本研究有助于更好地理解有益根际细菌、真菌病原菌和宿主植物之间相互作用的机制。
