Fritz G, Rahmsdorf H J, Kaina B
Kernforschungszentrum Karlsruhe, Institut für Genetik und für Toxikologie von Spaltstoffen, F.R.G.
Mutat Res. 1990 May;244(1):49-53. doi: 10.1016/0165-7992(90)90107-u.
Chinese hamster ovary (CHO-9) and HeLa MR cells lack detectable amounts of O6-alkylguanine DNA alkyltransferase (phenotypically Mex-) and are hypersensitive to the toxic effect of N-hydroxyethyl-N-chloroethylnitrosourea (HeCNU), as compared to Mex+ derivatives. Microinjection of size-fractionated polyA+ mRNA extracted from HeLa S3 (Mex+) into CHO-9 and HeLa MR cells, or from ataxia telangiectasia (Mex+) into HeLa MR cells, gave rise to an increase in survival following treatment with toxic doses of HeCNU. Transient complementation of the Mex- phenotype was achieved with an RNA population 0.8-1 kb in size.
中国仓鼠卵巢细胞（CHO-9）和HeLa MR细胞缺乏可检测到的O6-烷基鸟嘌呤DNA烷基转移酶（表型为Mex-），并且与Mex+衍生物相比，对N-羟乙基-N-氯乙基亚硝基脲（HeCNU）的毒性作用高度敏感。将从HeLa S3（Mex+）提取的大小分级的聚腺苷酸加尾mRNA微注射到CHO-9和HeLa MR细胞中，或者将从共济失调毛细血管扩张症（Mex+）提取的mRNA微注射到HeLa MR细胞中，在用毒性剂量的HeCNU处理后，细胞存活率增加。用大小为0.8-1 kb的RNA群体实现了Mex-表型的瞬时互补。
