Lehmann E, Hänze J, Pauschinger M, Ganten D, Lang R E
German Institute for High Blood Pressure Research, Heidelberg.
Neurosci Lett. 1990 Mar 26;111(1-2):170-5. doi: 10.1016/0304-3940(90)90363-e.
Pro-vasopressin-neurophysin mRNA (AVP mRNA) was assayed in the pituitary gland of rats from different strains, using a specific cRNA probe corresponding to exon C of the vasopressin gene. Northern blot analysis revealed the presence of an about 650 base pair (bp) AVP mRNA in the neurolobe of all strains. No signal was found in the anterior or intermediate lobes. Partial nucleotide sequence analysis suggested identity with the genuine AVP mRNA. Osmotic stimulation with 2% saline or water deprivation increased AVP mRNA levels in a fashion that paralleled changes seen in the hypothalamus. The origin of the AVP mRNA in the neurolobe is uncertain but is likely to be the pituicytes. Transport of the mRNA into the nerve endings of hypothalamic supraoptic and paraventricular neurons is an alternative possibility.
使用与血管加压素基因外显子C对应的特异性cRNA探针，对不同品系大鼠的垂体中血管加压素-神经垂体素原mRNA（AVP mRNA）进行检测。Northern印迹分析显示，所有品系大鼠神经叶中均存在约650个碱基对（bp）的AVP mRNA。在前叶或中间叶未发现信号。部分核苷酸序列分析表明其与真正的AVP mRNA相同。用2%盐水进行渗透刺激或禁水会使AVP mRNA水平升高，其方式与下丘脑所见变化平行。神经叶中AVP mRNA的来源尚不确定，但可能是垂体细胞。mRNA转运至下丘脑视上核和室旁核神经元的神经末梢是另一种可能。
