Department of Chemistry, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, USA.
Small. 2013 Jun 24;9(12):2189-98. doi: 10.1002/smll.201201798. Epub 2013 Jan 30.
Silica nanorods (SNRs) are synthesized and then functionalized with aminoalkoxysilanes to prepare a new class of nitric oxide (NO)-releasing materials. The aspect ratio and size of the SNRs are tuned by varying the temperature, pH, and silane concentration used during the surfactant-templated synthesis. N-Diazeniumdiolate nitric oxide (NO) donors are formed on the secondary amine-functionalized SNRs by reaction with NO gas under basic conditions. Particle surface modifications are employed to manipulate the NO release kinetics. The diverse morphology (i.e., aspect ratio ∼1-8), NO-release kinetics (2000-14,000 ppb NO/mg particle) and similar sizes (i.e., particle volume ∼0.02 μm³) of the resulting NO-releasing SNRs facilitates further studies of how particle shape and NO flux impacts bactericidal activity against Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Pseudomonas aeruginosa (P. aeruginosa) bacteria. The bactericidal efficacies of these materials improves with increasing particle aspect ratio and initial NO flux. Both chemical (i.e., NO-release kinetics) and physical (i.e., morphology) properties greatly influenced the bactericidal activity of these materials.
硅纳米棒（SNRs）被合成，然后用氨丙基硅烷进行功能化，以制备一类新型的一氧化氮（NO）释放材料。通过改变表面活性剂模板合成过程中使用的温度、pH 值和硅烷浓度，可以调整 SNRs 的纵横比和尺寸。在碱性条件下，通过与 NO 气体反应，在二级胺功能化的 SNRs 上形成 N-二亚硝胺基一氧化氮（NO）供体。通过表面改性来控制 NO 释放动力学。所得的具有不同形态（例如，纵横比约为 1-8）、NO 释放动力学（2000-14000 ppb NO/mg 颗粒）和相似尺寸（例如，颗粒体积约为 0.02 μm³）的 NO 释放 SNRs，便于进一步研究颗粒形状和 NO 通量如何影响对革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性铜绿假单胞菌（P. aeruginosa）细菌的杀菌活性。这些材料的杀菌功效随颗粒纵横比和初始 NO 通量的增加而提高。这些材料的杀菌活性受到化学性质（即 NO 释放动力学）和物理性质（即形态）的极大影响。
