Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
ACS Nano. 2011 Sep 27;5(9):7235-44. doi: 10.1021/nn202054f. Epub 2011 Aug 29.
A reverse microemulsion synthesis was used to prepare amine-functionalized silica nanoparticles of three distinct sizes (i.e., 50, 100, and 200 nm) with similar amine content. The resulting hybrid nanoparticles, consisting of N-(6-aminohexyl)aminopropyltrimethoxysilane and tetraethoxysilane, were highly monodisperse in size. N-Diazeniumdiolate nitric oxide (NO) donors were subsequently formed on secondary amines while controlling reaction conditions to keep the total amount of NO released constant for each particle size. The bactericidal efficacy of the NO-releasing nanoparticles against Pseudomonas aeruginosa increased with decreasing particle size. Additionally, smaller diameter nanoparticles were found to associate with the bacteria at a faster rate and to a greater extent than larger particles. Neither control (non-NO-releasing) nor NO-releasing particles exhibited toxicity toward L929 mouse fibroblasts at concentrations above their respective minimum bactericidal concentrations. This study represents the first investigation of the bactericidal efficacy of NO-releasing silica nanoparticles as a function of particle size.
采用反相微乳液法合成了三种不同尺寸（50nm、100nm 和 200nm）的胺功能化硅纳米粒子，其胺含量相似。所得的杂化纳米粒子由 N-(6-氨基己基)氨丙基三甲氧基硅烷和四乙氧基硅烷组成，粒径高度单分散。随后在仲胺上形成 N-二亚氨基二硝酸盐（NO）供体，同时控制反应条件使每个粒径释放的 NO 总量保持不变。释放 NO 的纳米粒子对铜绿假单胞菌的杀菌效果随粒径的减小而增加。此外，与较大粒径的颗粒相比，较小直径的颗粒更快地与细菌结合，并且结合程度更高。在高于各自最小杀菌浓度的浓度下，对照（非 NO 释放）和 NO 释放颗粒对 L929 小鼠成纤维细胞均无毒性。本研究首次研究了作为粒径函数的释放 NO 的硅纳米粒子的杀菌功效。
