Orth D N, Guillemin R, Ling N, Nicholson W E
J Clin Endocrinol Metab. 1978 May;46(5):849-52. doi: 10.1210/jcem-46-5-849.
The immunoreactive (RIA), ACTH, LPH, alpha endorphin (alpha End) and beta End in an extract of a human pancreatic islet cell carcinoma causing the ectopic ACTH syndrome were assayed after gel exclusion chromatography. In addition to "big", "intermediate" and "little" ACTHs, beta lipotropin (beta LPH) and gamma LPH, the eluate fractions contained RIA-alpha End and -beta End. The major RIA-beta End component appeared to be h beta LPH, which has beta End as its carboxy-terminus, but significant concentrations of a component that coeluted with synthetic p beta End were also found. Small amounts of RIA-alpha End were found in two peaks, one that may have represented 1-76 h beta LPH, with alpha End as its carboxy-terminus, and one probably representing alpha End itself. RIA-ACTH, -LPH and -beta End were found in the void volume fractions. Thus, a human nonpituitary tumor that produced ACTH and LPHs also produced endorphins, all perhaps deriving from a common precursor.
对一例导致异位促肾上腺皮质激素（ACTH）综合征的人胰岛细胞瘤提取物，经凝胶排阻色谱法分离后，测定了其中的免疫反应性（放射免疫测定法，RIA）ACTH、促脂解素（LPH）、α-内啡肽（α-End）和β-内啡肽。除了“大分子”、“中分子”和“小分子”ACTH、β-促脂解素（β-LPH）和γ-LPH外，洗脱组分中还含有RIA-α-End和-β-End。主要的RIA-β-End组分似乎是hβ-LPH，其羧基末端为β-End，但也发现了与合成的pβ-End共洗脱的一种组分的显著浓度。在两个峰中发现了少量的RIA-α-End，一个峰可能代表1-76 hβ-LPH，其羧基末端为α-End，另一个峰可能代表α-End本身。RIA-ACTH、-LPH和-β-End在空体积组分中被发现。因此，一个产生ACTH和LPHs的人非垂体肿瘤也产生内啡肽，所有这些可能都源自一个共同的前体。
