Veterinary Integrated Research Unit, Faculty of Sciences, Namur Research Institute for Life Sciences (NARILIS), University of Namur (FUNDP), 5000 Namur, Belgium.
J Gen Virol. 2013 Jun;94(Pt 6):1168-1174. doi: 10.1099/vir.0.051821-0. Epub 2013 Jan 30.
Detected for the first time in 2011, Schmallenberg virus (SBV) is an orthobunyavirus of the Simbu serogroup that caused a large outbreak in European ruminants. In a tight time frame, data have been obtained on SBV epidemiology and the clinical pictures associated with this new viral infection, but little information is available on the molecular biology of SBV. In this study, SBV sequence variability was characterized from the central nervous system of two stillborn lambs in a naturally infected herd. A hypervariable region (HVR) was detected in the N-terminal region of the SBV Gc glycoprotein through sequencing and analysis of the two full-length genomes representative of intra-herd SBV dissemination. In vitro growth assays coupled with full-length genome sequencing were performed on the two isolates after successive cellular passages, showing an in vitro adaptation of SBV and mutation accumulation inside the HVR in the absence of immune selective pressure.
沙氏山病毒（SBV）于 2011 年首次被发现，是一种属于辛巴威血清群的正呼肠孤病毒，曾在欧洲反刍动物中引发大规模疫情。在时间紧迫的情况下，人们已经获得了关于 SBV 流行病学和与之相关的临床图片的相关数据，但关于 SBV 的分子生物学信息还很有限。在这项研究中，从一个自然感染的羊群中两只死胎羔羊的中枢神经系统中对 SBV 序列变异性进行了研究。通过对代表羊群内部 SBV 传播的两个全长基因组的测序和分析，在 SBV Gc 糖蛋白的 N 端区域检测到一个高变区（HVR）。对两个分离株进行了连续细胞传代的体外生长试验和全长基因组测序，结果表明 SBV 在体外适应，并且在没有免疫选择压力的情况下，HVR 内发生了突变积累。
