Palatnik C B, Previato J O, Mendonça-Previato L, Borojevic R
Departamento de Parasitologia, Universidade Federal do Rio de Janeiro, Brazil.
Parasitol Res. 1990;76(4):289-93. doi: 10.1007/BF00928181.
Two Leishmania donovani glycoconjugate ligands for the internalization receptor on BALB/c peritoneal macrophages [fucose-mannose ligand (FML) and phosphate mannogalactan ligand (PMGL)] were shown to be species-specific in a comparative phagocytosis-inhibition test. Promastigotes of L. donovani Sudan (LD1S), L. infantum, L. d. donovani, L. major (Jericho and Sudan), L. tropica, L. chagasi, L. mexicana venezuelensis, L. m. mexicana, L. m. amazonensis, L. m. pifanoi, L. m. garnhami, L. braziliensis braziliensis, L. m. amazonensis (Josefa), L. enrietti or L. adleri were incubated with macrophages in the presence of 10 micrograms/ml FML and PMGL purified from L. donovani (LD1S). Parasite internalization was determined and compared with that obtained in control experiments. Specific inhibition of phagocytosis ranged from 83% (L. donovani LD1S) to 7% (L. m. amazonensis). We could distinguish groups of Leishmania consistently with their geographic distribution and the clinical aspects of the disease. Analogous experiments with L. m. amazonensis glycoconjugates showed reciprocal results, with inhibition ranging from 76% (L. m. amazonensis) to 8% (L. donovani LD1S). L. chagasi remained separated from the Old World kala-azar agents. Possible phylogenetic implications of these observations are discussed.
在一项比较吞噬抑制试验中，已证明杜氏利什曼原虫的两种用于BALB/c腹膜巨噬细胞内化受体的糖缀合物配体（岩藻糖-甘露糖配体（FML）和磷酸甘露半乳聚糖配体（PMGL））具有种特异性。将杜氏利什曼原虫苏丹株（LD1S）、婴儿利什曼原虫、杜氏利什曼原虫指名亚种、硕大利什曼原虫（耶利哥株和苏丹株）、热带利什曼原虫、恰加斯利什曼原虫、委内瑞拉利什曼原虫、墨西哥利什曼原虫指名亚种、亚马逊利什曼原虫、皮法诺利什曼原虫、加恩哈米利什曼原虫、巴西利什曼原虫巴西亚种、亚马逊利什曼原虫（约瑟法株）、恩氏利什曼原虫或阿氏利什曼原虫的前鞭毛体与巨噬细胞在存在从杜氏利什曼原虫（LD1S）纯化的10微克/毫升FML和PMGL的情况下孵育。测定寄生虫的内化情况并与对照实验中获得的结果进行比较。吞噬作用的特异性抑制范围从83%（杜氏利什曼原虫LD1S）到7%（亚马逊利什曼原虫）。我们可以根据其地理分布和疾病的临床方面一致地区分利什曼原虫群体。用亚马逊利什曼原虫糖缀合物进行的类似实验显示了相反的结果，抑制范围从76%（亚马逊利什曼原虫）到8%（杜氏利什曼原虫LD1S）。恰加斯利什曼原虫与旧世界黑热病病原体仍有区别。讨论了这些观察结果可能的系统发育意义。
