一系列利什曼原虫物种的糖缀合物的凝集素结合特性比较。

A comparison of the lectin-binding properties of glycoconjugates from a range of Leishmania species.

作者信息

Rossell R J, Stevens A F, Miles M A, Allen A K

机构信息

Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK.

出版信息

Parasitol Res. 1990;76(4):294-300. doi: 10.1007/BF00928182.

DOI:10.1007/BF00928182

PMID:2336446

Abstract

Constituent glycoconjugates of promastigotes of 14 different Leishmania strains from 6 different species were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and subsequently stained with 14 125I-labelled lectins of different specificities. Autoradiography of the gels revealed lectin-specific glycoproteins and other glycoconjugates of known molecular weight. Similarities and differences in antigens and glycoproteins present in the strains are described. The possibility of identification or characterisation of Leishmania species from their electrophoretic behaviour and lectin-binding patterns is unlikely, but these results should be an aid to purification of glycoconjugates from particular strains by lectin-affinity chromatography.

摘要

来自6个不同物种的14种不同利什曼原虫菌株的前鞭毛体的组成性糖缀合物，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）进行分离，随后用14种不同特异性的125I标记凝集素进行染色。凝胶的放射自显影片显示了凝集素特异性糖蛋白和其他已知分子量的糖缀合物。描述了这些菌株中存在的抗原和糖蛋白的异同。根据其电泳行为和凝集素结合模式来鉴定或表征利什曼原虫物种的可能性不大，但这些结果应有助于通过凝集素亲和色谱法从特定菌株中纯化糖缀合物。